2020年11月23日
Structural and dynamics analysis of intrinsically disordered proteins by high-speed atomic force microscopy.
Nature nanotechnology
- 巻
- 16
- 号
- 2
- 開始ページ
- 181
- 終了ページ
- 189
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41565-020-00798-9
Intrinsically disordered proteins (IDPs) are ubiquitous proteins that are disordered entirely or partly and play important roles in diverse biological phenomena. Their structure dynamically samples a multitude of conformational states, thus rendering their structural analysis very difficult. Here we explore the potential of high-speed atomic force microscopy (HS-AFM) for characterizing the structure and dynamics of IDPs. Successive HS-AFM images of an IDP molecule can not only identify constantly folded and constantly disordered regions in the molecule, but can also document disorder-to-order transitions. Moreover, the number of amino acids contained in these disordered regions can be roughly estimated, enabling a semiquantitative, realistic description of the dynamic structure of IDPs.
- リンク情報
- ID情報
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- DOI : 10.1038/s41565-020-00798-9
- PubMed ID : 33230318