In our previous study, the decrease of glutathione and the increase of four inflammatory cytokines in blood were identified as nongenetic risk factors of drug induced liver injury (DILI) in patients. In this study, cell toxicity was evaluated using rat primary isolated hepatocytes in the presence and the absence of these risk factors for 223 drugs, and it was revealed that the ratio correlated well with the observed risk of DILI in human. This method was superior to the standard method based on the amount of protein adducts, and would be useful for new drug development in the near future.