2022年5月16日
Novel electron microscopic staining method using traditional dye, hematoxylin.
Scientific reports
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- 巻
- 12
- 号
- 1
- 開始ページ
- 7756
- 終了ページ
- 7756
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41598-022-11523-y
Uranyl acetate (UA) has been routinely used as a staining solution for ultrathin sections used in biological electron microscopy. As a radioactive nuclear material, UA is subject to strict international regulations. To develop an alternative and easy-to-use staining method for ultrathin sections, we examined various commercial light microscopic dyes. We found that Mayer's hematoxylin followed by Reynold's lead citrate solution showed staining results comparable to UA and Reynold's lead citrate solution, and this method is therefore suggested as a reliable and promising alternative to UA staining.
- リンク情報
- ID情報
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- DOI : 10.1038/s41598-022-11523-y
- PubMed ID : 35577902
- PubMed Central 記事ID : PMC9110702