1999年10月
Intramolecular quadruplex formation of the G-rich strand of the mouse hypervariable minisatellite Pc-1
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- 巻
- 264
- 号
- 2
- 開始ページ
- 327
- 終了ページ
- 333
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1006/bbrc.1999.1521
- 出版者・発行元
- ACADEMIC PRESS INC
The minisatellite Pc-1, isolated from the mouse genome consisting of a tandem repeat of d(GGCAG), is hypervariable with a mutation rate of 0.15/generation. Here we describe a structural characterization of the G-rich strand of Pc-1 by biochemical and physicochemical methods. It was found to be comparatively resistant to both single-stranded DNA-binding protein binding and digestion by single-stranded DNA-specific nuclease and to cause arrest of DNA synthesis. The guanine imino proton NMR signals observed on the Pc-1 G-rich strand and their slow H-1/H-2 exchange profiles pointed to a quadruplex structure with guanine quartets. The melting temperature of the quadruplex determined by CD was not dependent on DNA concentration. These results indicate that the G-rich strand of Pc-1 forms an intramolecular folded-back quadruplex structure under physiological conditions. Possible mechanisms of the Pc-1 mutations implicated with the formation of the quadruplex structure are discussed. (C) 1999 Academic Press.
- リンク情報
- ID情報
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- DOI : 10.1006/bbrc.1999.1521
- ISSN : 0006-291X
- Web of Science ID : WOS:000083351400004