2009年6月
Structural Studies of an RNA Aptamer Containing GGA Repeats Under Ionic Conditions Using Microchip Electrophoresis, Circular Dichroism, and 1D-NMR
OLIGONUCLEOTIDES
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- 巻
- 19
- 号
- 2
- 開始ページ
- 179
- 終了ページ
- 189
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1089/oli.2008.0167
- 出版者・発行元
- MARY ANN LIEBERT INC
Nuclear magnetic resonance (NMR) studies have shown that RNA/DNA oligomers with GGA repeat sequences contain unique G-quadruplex structures in the presence of K(+) or Na(+) ions. In this study, we used microchip electrophoresis to study the structure of an RNA aptamer against bovine prion protein that possessed four GGA-triplet repeats (wt2). We analyzed the structural changes and characterized dimer formation of the aptamer. Mutational, circular dichroism, and one-dimensional NMR studies of wt2 revealed that K(+) ions induce wt2 to assume a thermostable dimer in an intramolecular G-quadruplex with parallel orientation.
- リンク情報
- ID情報
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- DOI : 10.1089/oli.2008.0167
- ISSN : 1545-4576
- Web of Science ID : WOS:000266730100009