2010年3月
Solubilization and structural determination of a glycoconjugate which is assembled into the sheath of Leptothrix cholodnii
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 46
- 号
- 2
- 開始ページ
- 206
- 終了ページ
- 211
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ijbiomac.2009.12.006
- 出版者・発行元
- ELSEVIER SCIENCE BV
The sheath of Leptothrix cholodnii is constructed from a structural glycoconjugate, a straight-chained amphoteric heteropolysaccharide modified with glycine and cysteine. Though the structure of the glycan core is already determined, its modifications with amino acids and other molecules are not fully resolved In this study, we aimed to determine the chemical structure of the glycoconjugate as a whole Enantiomeric determination of cysteine in the sheath was performed and as a result. L-cysteine was detected. NMR spectroscopy was endeavored to determine overall structure of the glycoconjugate. Prior to NMR analysis, solubilization of the glycoconjugate was attempted by adding denaturing reagents or by derivatization As far as tested, sulfonation by performic acid oxidation was suitable for solubilization, but further improvement was achieved by N-acetylation. The approximate molecular weight of the derivative was estimated to be 4.5 x 10(4) by size-exclusion chromatography The NMR studies for the sulfonated glycoconjugate and its N-acetylated derivative revealed that the sheath glycoconjugate is a glycosaminoglycan consisting of a pentasaccharide repeating unit which is substoichiometrically esterified with 3-hydroxypropionic acid and stoichiometrically amidated with acetic acid and glycyl-L-cysteine. (C) 2009 Elsevier B V All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.ijbiomac.2009.12.006
- ISSN : 0141-8130
- Web of Science ID : WOS:000275700900012