論文

査読有り
2016年12月

Development of an ATP and hydrogen ion image sensor using a patterned apyrase-immobilized membrane

TALANTA
ダウンロード
回数 : 183
  • You-Na Lee
  • ,
  • Koichi Okumura
  • ,
  • Tatsuya Iwata
  • ,
  • Kazuhiro Takahashi
  • ,
  • Toshiaki Hattori
  • ,
  • Makoto Ishida
  • ,
  • Kazuaki Sawada

161
開始ページ
419
終了ページ
424
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.talanta.2016.08.070
出版者・発行元
ELSEVIER SCIENCE BV

A bio-image sensor using a patterned apyrase-immobilized membrane was developed to visualize the activities of adenosine triphosphate (ATP) and H+ ion in real-time. An enzymatic membrane patterning technique was suggested to immobilize apyrase on a specific sensing area of a charge coupled device (CCD)-type image sensor. It was able to observe the spatiotemporal information of ATP and H+ ion. The smallest size of a patterned membrane is 250 x 250 mu m(2). The fabrication parameters of the patterned membrane, such as its thickness and the intensity of the incident light used for photolithography, were optimized experimentally. The sensing area under the patterned apyrase-immobilized membrane revealed a linear response up to 0.6 mM of ATP concentration with a sensitivity of 37.8 mV/mM. Meanwhile, another sensing area without the patterned membrane measured the diffused H+ ion from nearby membranes. This diffusion characteristics were analyzed to determine a measurement time that can minimize the undesirable impact of the diffused ions. In addition, the newly developed bio-image sensor successfully reconstructed ATP and H+ ion dynamics into sequential 2-dimensional images. (C) 2016 Elsevier B.V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.talanta.2016.08.070
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000386989500053&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.talanta.2016.08.070
  • ISSN : 0039-9140
  • eISSN : 1873-3573
  • Web of Science ID : WOS:000386989500053

エクスポート
BibTeX RIS