山口陽子 (Yoko Yamaguchi) - マイポータル

基本情報

所属: 順天堂大学大学院医学研究科客員教授

学位: 農学博士(東京大学)

通称等の別名: FUJITA-YAMAGUCHI Yoko
J-GLOBAL ID: 200901051946750885
researchmap会員ID: 1000316839

外部リンク: http://www.cityofhope.org/people/fujita-yamaguchi-yoko

アメリカ、カリフォルニア州ロスアンゼルス郊外のCity of Hope Beckman研究所にポスドク3年後、20年間PIとして研究活動をしたのち、2000年から東海大学工学部生命化学科で研究・教育を14年勤めました。2014年4月からCity of Hopeに復帰し、研究の継続をしつつ、日米の研究・教育交流を高校生、学部生、院生、ポスドク、研究者の各レベルで推進しています。日本では、理研の客員研究員として共同研究を進めています。

研究キーワード

研究分野

経歴

2022年5月 - 現在

順天堂大学, 大学院医学研究科, 客員教授
2014年4月 - 現在

City of Hope ベックマン研究所, Diabetes & Metabolism Res. Inst., Professor Emeritus
2000年 - 2014年3月

東海大学工学部生命化学科教授 & 米国City of Hopeベックマン研究所Adjunct Professor of Molecular Biology
1981年 - 2000年

米国City of Hopeベックマン研究所Assistant ProfessorからProfessor of Molecular Biology
1981年 - 2000年

Assistant Professor to Professor of Molecular Biology at Beckman Research Institute of the City of Hope
2000年

- Professor of Applied Biochemistry, School of Engineering, Tokai University & Adjunct Professor of Molecular Biology at Beckman Research Institute of the City of Hope
1977年 - 1981年

米国City of Hope研究所博士研究員
1977年 - 1981年

Research Fellow at City of Hope Medical Center

学歴

論文

Proteolytic Processing, Maturation, and Unique Synteny of the Streptomyces Hemagglutinin SHA.

Yoko Fujita-Yamaguchi, Hideyuki Muramatsu, Alonso Tapia, Karine Bagramyan, Moksha Desai, Yasuhiro Takehana, Masayuki Igarashi, Yoshiki Yamaguchi, Markus Kalkum

Microbiology spectrum 9(2) e0076621 2021年9月1日
MLS128 antibody-induced suppression of colon cancer cell growth is mediated by a desmocollin and a 110 kDa glycoprotein.

Sarah C Shuck, Teresa Hong, Markus Kalkum, Ryo Igarashi, Kota Kajiya, John Termini, Kazuo Yamamoto, Yoko Fujita-Yamaguchi

Bioscience trends 13(3) 216-224 2019年7月22日
Local structural changes of the influenza A virus ribonucleoprotein complex by single mutations in the specific residues involved in efficient genome packaging.

Naoki Takizawa, Yoshitoshi Ogura, Yoko Fujita, Takeshi Noda, Hideki Shigematsu, Tetsuya Hayashi, Ken Kurokawa

Virology 531 126-140 2019年5月査読有り
Mass spectrometric revival of an l-rhamnose- and d-galactose-specific lectin from a lost strain of Streptomyces.

Fujita-Yamaguchi Y, Bagramyan K, Yamaguchi Y, Ikeda A, Dohmae N, Hong TB, Kalkum M

The Journal of biological chemistry 293(1) 368-378 2018年1月査読有り
An aqueous extract from toad skin prevents gelatinase activities derived from fetal serum albumin and serum-free culture medium of human breast carcinoma MDA-MB-231 cells.

Nakata M, Kawaguchi S, Oikawa A, Inamura A, Nomoto S, Miyai H, Nonaka T, Ichimi S, Fujita-Yamaguchi Y, Luo C …

Drug discoveries & therapeutics 9(6) 417-421 2016年査読有り
Knockdown of AT-rich interaction domain (ARID) 5B gene expression induced AMPKα2 activation in cardiac myocytes.

Hirose-Yotsuya L, Okamoto F, Yamakawa T, Whitson RH, Fujita-Yamaguchi Y, Itakura K

Bioscience trends 9(6) 377-385 2015年12月査読有り
Affinity chromatography of native and recombinant proteins from receptors for insulin and IGF-I to recombinant single chain antibodies

Yoko Fujita-Yamaguchi

FRONTIERS IN ENDOCRINOLOGY 6 166 2015年10月査読有り
Toad skin extract cinobufatini inhibits migration of human breast carcinoma MDA-MB-231 cells into a model stromal tissue

Munehiro Nakata, Shuya Mori, Yo Kamoshida, Shota Kawaguchi, Yoko Fujita-Yamaguchi, Bo Gao, Wei Tang

BIOSCIENCE TRENDS 9(4) 266-269 2015年8月査読有り
Susceptibility to proteases of anti-Tn-antigen MLS128 binding glycoproteins expressed in human colon cancer cells

Fumie Oura, Yukiko Yajima, Munehiro Nakata, Kenzui Taniue, Tetsu Akiyama, Hiroshi Nakada, Kazuo Yamamoto, Yoko Fujita-Yamaguchi

BIOSCIENCE TRENDS 9(1) 49-55 2015年2月査読有り
Migration of breast cancer cells into reconstituted type I collagen gels assessed via a combination of frozen sectioning and azan staining

Kyohei Fukuda, Yo Kamoshida, Taisuke Kurokawa, Mioto Yoshida, Yoko Fujita-Yamaguchi, Munehiro Nakata

BIOSCIENCE TRENDS 8(4) 212-216 2014年8月査読有り
Purification and refolding of anti-T-antigen single chain antibodies (scFvs) expressed in Escherichia coli as inclusion bodies

Noriyuki Yuasa, Tsubasa Koyama, Yoko Fujita-Yamaguchi

BIOSCIENCE TRENDS 8(1) 24-31 2014年査読有り
Expression and structural characterization of anti-T-antigen single-chain antibodies (scFvs) and analysis of their binding to T-antigen by surface plasmon resonance and NMR spectroscopy

Noriyuki Yuasa, Tsubasa Koyama, Ganesh P. Subedi, Yoshiki Yamaguchi, Misao Matsushita, Yoko Fujita-Yamaguchi

JOURNAL OF BIOCHEMISTRY 154(6) 521-529 2013年12月査読有り
Renewed interest in basic and applied research involving monoclonal antibodies against an oncofetal Tn-antigen

Yoko Fujita-Yamaguchi

JOURNAL OF BIOCHEMISTRY 154(2) 103-105 2013年8月査読有り
Characterization of anti-Tn-antigen MLS128 binding proteins involved in inhibiting the growth of human colorectal cancer cells

Normaiza Zamri, Naoya Masuda, Fumie Oura, Kazuya Kabayama, Yukiko Yajima, Hiroshi Nakada, Kazuo Yamamoto, Yoko Fujita-Yamaguchi

BioScience Trends 7(5) 221-229 2013年査読有り
Surface plasmon resonance and NMR analyses of anti Tn-antigen MLS128 monoclonal antibody binding to two or three consecutive Tn-antigen clusters.

Matsumoto-Takasaki A, Hanashima S, Aoki A, Yuasa N, Ogawa H, Sato R, Kawakami H, Mizuno M, Nakada H, Yamaguchi Y …

Journal of biochemistry 151(3) 273-282 2012年3月査読有り
Overproduction of anti-Tn antibody MLS128 single-chain Fv fragment in Escherichia coli cytoplasm using a novel pCold-PDI vector.

Subedi GP, Satoh T, Hanashima S, Ikeda A, Nakada H, Sato R, Mizuno M, Yuasa N, Fujita-Yamaguchi Y, Yamaguchi Y

Protein expression and purification 82(1) 197-204 2012年3月査読有り
NMR Analysis of an Anti-Carbohydrate Antibody MLS128 Single-Chain Fv Fragment toward Elucidation of the Multivalent Recognition Mechanism

Ganesh Prasad Subedi, Tadashi Satoh, Shinya Hanashima, Akemi Ikeda, Hiroshi Nakada, Reiko Sato, Mamoru Mizuno, Noriyuki Yuasa, Yoko Fujita-Yamaguchi, Yoshiki Yamaguchi

GLYCOBIOLOGY 21(11) 1480-1480 2011年11月査読有り
Characterization of three different single chain antibodies recognizing non-reducing terminal mannose residues expressed in Escherichia coli by an inducible T7 expression system

Ayano Matsumoto-Takasaki, Noriyuki Yuasa, Daiichi Katagiri, Tsubasa Koyama, Keiko Sakai, Normaiza Zamri, Sheryl Phung, Shiuan Chen, Hiroshi Nakada, Munehiro Nakata …

JOURNAL OF BIOCHEMISTRY 150(4) 439-450 2011年10月査読有り
Affinity of anti-insulin-like growth factor I receptor antibody binding to the receptor altered by plant lectins

Yu Kusada, Yoko Fujita-Yamaguchi

BIOSCIENCE TRENDS 5(3) 93-98 2011年査読有り
Invasion of carcinoma cells into reconstituted type I collagen gels: Visual real-time analysis by time-lapse microscopy

Keiko Sakai, Taisuke Kurokawa, Yoshihiro Furui, Yuh Kuronuma, Masaru Sekiguchi, Jun Ando, Yoshinori Inagaki, Wei Tang, Munehiro Nakata, Yoko Fujita-Yamaguchi

BIOSCIENCE TRENDS 5(1) 10-16 2011年査読有り

もっとみる

MISC

165

Primary structure determination of a blood group B-specific lectin purified from Streptomyces sp 27S5 reveals insight into its mechanism of expression and unique structural features

Yoko Fujita-Yamaguchi, Yoshiki Yamaguchi, Akemi Ikeda, Naoshi Domae, Karine Bagramyan, Teresa B. Hong, John P. Murad, Markus Kalkum

GLYCOBIOLOGY 26(12) 1415-1415 2016年12月
Proteomics and Genomics Provide Novel Insights Into Streptomyces Lectin Biochemistry

Markus Kalkum, Teresa Hong, Karine Bagramyan, Yoko Fujita-Yamaguchi

GLYCOBIOLOGY 24(11) 1115-1116 2014年11月
Expression and Purification of Anti-IGF-I Receptor 1H7 Constructs in Drosophila S2 Cells

Yoko Fujita-Yamaguchi, Astufumi Nakazawa, Noriyuki Yuasa

ENDOCRINE REVIEWS 35(3) 2014年6月
Expression, purification, and refolding of single chain antibodies (scFvs) from inclusion bodies

Hiroyuki Kato, Takahito Fukuda, Noriyuki Yuasa, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 244 2012年8月
In vitro production of anticarbohydrate antibodies: Purification of anti-T antigen antibodies (scFvs) from E. coli inclusion bodies and Drosophila S2 cell culture media

Tsubasa Koyama, Daisuke Takada, Yoshiki Yamaguchi, Noriyuki Yuasa, Ayano Matsumoto-Takasaki, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 244 2012年8月
Effects of T-antigen specific peanut agglutinin (PNA) on LS180 colon cancer cell growth: Potential interaction with MLS128 monoclonal antibody against Tn-antigen

Fumie Oura, Keito Iijima, Yukiko Yajima, Hiroshi Nakada, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 244 2012年8月
Purification of anti-insulin-like growth factor-I receptor (IGF-IR) single chain antibody (scFv) expressed in Drosophila S2 cells

Atsufumi Nakazawa, Noriyuki Yuasa, Anano Matsumoto-Takasaki, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 244 2012年8月
Effects of anti-insulin-like growth factor-I receptor (IGF-IR) antibodies with or without the ER-retention signal KDEL on cancer cell growth

Yu-Ming Guo, Noriyuki Yuasa, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 244 2012年8月
Effects of two monoclonal antibodies, MLS128 against Tn-antigen and 1H7 against insulin-like growth factor-I receptor, on the growth of colon cancer cells.

Zamri N, Masuda N, Oura F, Yajima Y, Nakada H, Fujita-Yamaguchi Y

Bioscience Trends 6(6) 303-312 2012年
Construction and expression of anti-Tn-antigen-specific single-chain antibody genes from hybridoma producing MLS128 monoclonal antibody.

Yuasa N, Ogawa H, Koizumi T, Tsukamoto K, Matsumoto-Takasaki A, Asanuma H, Nakada H, Fujita-Yamaguchi Y

Journal of Biochemistry 151(4) 371-378 2012年
Effects of monoclonal antibodies against Tn antigen (MLS128) and insulin-like growth factor (IGF)-I receptor (1H7) on colon cancer cell growth

Normaiza Zamri, Naoya Masuda, Fumie Oura, Yukiko Yajima, Hiroshi Nakada, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 241 2011年3月
Purification and characterization of anti-LNFPIII single chain antibody (scFv) conjugated with Fc from CHO cells transfected with 3F1 scFv-Fc gene

Yasunobu Tsuchida, Kaname Funayama, Daisuke Kubo, Noriyuki Yuasa, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 241 2011年3月
In vitro production of anticarbohydrate antibodies: Purification of antitrimannose single chain antibodies (scFvs) from inclusion bodies

Daiichi Katagiri, Tubasa Koyama, Ayano Matsumoto-Takasaki, Noriyuki Yuasa, Keiko Sakai, Munehiro Nakata, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 241 2011年3月
Expression and purification of single chain antibodies (scFvs) isolated from a phage-displayed library with LNFPIII (Le(x)) probe

Hiroyuki Kato, Haruhiko Ogawa, Masato Takano, Daiichi Katagiri, Kotaro Kawamoto, Noriyuki Yuasa, Ayano Matsumoto-Takasaki, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 241 2011年3月
"US-JAPAN CONFERENCE: INFLAMMATION, DIABETES AND CANCER" held at the Beckman Research Institute of City of Hope, Duarte, CA, USA

Yoko Fujita-Yamaguchi, Ren-Jang Lin, Richard Jove

BIOSCIENCE TRENDS 5(6) 277-280 2011年
Construction and expression of anti-Tn-antigen (alpha GalNAc-Ser/Thr)-specific MLS128 single chain antibody (scFv) genes

Haruhuko Ogawa, Noriyuki Yuasa, Yu Kusada, Hideki Asanuma, Ayano Takasaki-Matsumoto, Yoko Fujita-Yamaguchi

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 240 2010年8月
Preparation of asialo-agalacto-glycophorin A for screening of anti-Tn antibodies.

Koizumi T, Matsumoto-Takasaki A, Nakada H, Nakata M, Fujita-Yamaguchi Y

Bioscience Trends 4(6) 308-311 2010年
Mechanisms of antibody-mediated insulin-like growth factor I receptor (IGF-IR) down-regulation in MCF-7 breast cancer cells

Masahiro Ohtani, Maki Numazaki, Yukiko Yajima, Yoko Fujita-Yamaguchi

BIOSCIENCE TRENDS 3(4) 131-138 2009年
Isolation and characterization of anti-T-antigen single chain antibodies from a phage library

Ayano Matsumoto-Takasaki, Jinichiro Horie, Keiko Sakai, Yoshihiro Furui, Reiko Sato, Hiroko Kawakami, Kazunori Toma, Atsushi Takayanagi, Nobuyoshi Shimizu, Yoko Fujita-Yamaguchi

BIOSCIENCE TRENDS 3(3) 87-95 2009年
Inhibition of cancer cell growth by anti-Tn monoclonal antibody MLS128

Noriyuki Morita, Yukiko Yajima, Hideki Asanuma, Hiroshi Nakada, Yoko Fujita-Yamaguchi

BIOSCIENCE TRENDS 3(1) 32-37 2009年

もっとみる

書籍等出版物

Isolation and characterization of antibodies against three consecutive Tn-antigen clusters from a phage library displaying human single-chain variable fragments.

J. Biochem. (Sakai, K., Yuasa, N., Tsukamoto, K., Takasaki-Matsumoto, A., Yajima, Y., Sato, R., Kawakami, H., Mizuno, M., Takayanagi, A., Shimizu, N., Nakata, M., and Fujita-Yamaguchi, Y. ) 2010年
Preparation of asialo-agalacto-glycophorin A for screening of anti-Tn antibodies.

Biosci. Trends. Koizumi, T., Matsumoto-Takasaki, A., Nakada, H., Nakata, M., Fujita-Yamaguchi, Y. 2010年
Production of anti-carbohydrate antibodies by phage-display technologies: Potential impairment of cell growth as a result of endogenous expression.

J. Biol. Chem. (Yuasa, N., Zhang, W., Goto, T., Sakaue, H., Matsumoto- Takasaki, A., Kimura, M., Ohshima, H., Tsuchida, Y., Koizumi, T., Sakai, K., Kojima, T., Yamamoto, K., Nakata, M., and Fujita-Yamaguchi, Y.) 2010年
Preparation of asialo-agalacto-glycophorin A for screening of anti-Tn antibodies.

Biosci. Trends. (Koizumi, T., Matsumoto-Takasaki, A., Nakada, H., Nakata, M., Fujita-Yamaguchi, Y.) 2010年
Production of anti-carbohydrate antibodies by phage-display technologies: Potential impairment of cell growth as a result of endogenous expression.

J. Biol. Chem. (110) Yuasa, N., Zhang, W., Goto, T., Sakaue, H., Matsumoto- Takasaki, A., Kimura, M., Ohshima, H., Tsuchida, Y., Koizumi, T., Sakai, K., Kojima, T., Yamamoto, K., Nakata, M., and Fujita-Yamaguchi, Y.) 2010年
Isolation and characterization of antibodies against three consecutive Tn-antigen clusters from a phage library displaying human single-chain variable fragments.

J. Biochem. ( Sakai, K., Yuasa, N., Tsukamoto, K., Takasaki-Matsumoto, A., Yajima, Y., Sato, R., Kawakami, H., Mizuno, M., Takayanagi, A., Shimizu, N., Nakata, M., and Fujita-Yamaguchi, Y.) 2010年

所属学協会

共同研究・競争的資金等の研究課題

新規糖鎖特異的単鎖抗体の大腸菌発現系による大量発現と糖鎖結合性解析

科学研究費補助金 2010年 - 2012年
Production of single chain antibodies (scFvs) against a variety of carbohydrate epitopes

JST Basic Research Programs (Core Research for Evolutional Science and Technology :CREST) 2003年 - 2009年
研究領域「糖鎖の生物機能の解明と利用技術」研究課題「糖鎖構造特異的単鎖抗体ライブラリーの構築」

ＪＳＴ戦略的創造研究推進制度（研究チーム型）（戦略的基礎研究推進事業：ＣＲＥＳＴ） 2003年 - 2008年
インスリン様増殖因子（IGF)-I 受容体とがん分子標的治療法

科学研究費補助金 2005年 - 2007年
Single chain antibodies against IGF-(]G0001[) receptor as potential therapeutics for cancer

Grant-in-Aid for Scientific Research 2005年 - 2007年
IGF-I receptor signalingをターゲットとしたがん分子標的治療法

その他の研究制度 1995年 - 2003年

山口陽子