2004年7月
Role of Nup98 in nuclear entry of human immunodeficiency virus type 1 cDNA
MICROBES AND INFECTION
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- 巻
- 6
- 号
- 8
- 開始ページ
- 715
- 終了ページ
- 724
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.micinf.2004.04.002
- 出版者・発行元
- ELSEVIER SCIENCE BV
Human immunodeficiency virus type 1 (HIV-1), like other lentiviruses, can infect non-dividing cells. The lentiviruses are most likely to have evolved a nuclear import strategy to import HIV-1 cDNA and viral protein complex through the nuclear pore complex (NPC) formed by nucleoporin proteins (Nup). In this study, we found that synthesis of integrated and 2LTR but not full-length form of HIV-1 cDNA was clearly impaired in culture via transduction of vesicular stomatitis virus matrix protein (VSV M), an inhibitor protein, through binding to the phenylalanine-glycine (FG) repeat region of Nup98. The impairment of synthesis of integrated and 2LTR DNA with VSV M was restored by ectopic overexpression of Nup98. A series of experiments using Nup98-depleted NPC by the small interfering RNA (siRNA) technique showed specific impairment of NPC structure and some functions, including nuclear import of HIV-1 cDNA. Our results suggest that Nup98 on the NPC specifically participates in the nuclear entry of HIV-1 cDNA following HIV-1 entry. (C) 2004 Elsevier SAS. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.micinf.2004.04.002
- ISSN : 1286-4579
- Web of Science ID : WOS:000222570700001