2001年4月
Expression, cloning, and immunological analysis of buckwheat (Fagopyrum esculentum Moench) seed storage proteins
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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- 巻
- 49
- 号
- 4
- 開始ページ
- 1825
- 終了ページ
- 1829
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/jf0011485
- 出版者・発行元
- AMER CHEMICAL SOC
cDNA of buckwheat (Fagopyrum esculentum Moench) was isolated from immature seeds harvested 14 days after pollination. Two genes, designated FA02 and FA18, were found:to encode legumin-like proteins and were expressed during seed development. The deduced amino acid sequence of FA02 was identical to the N-terminal amino acid domain of BW24KD, which was believed to be a major buckwheat allergen (Urisu, A.; Kondo, Y.; Morita, Y.; Yagi, E.; Tsuruta, M.; Yasaki, T.; Yamada, K.; Kuzuya, H.; Suzuki, M.; Titani, K.; Kurosawa, K. Isolation and characterization of a major allergen in buckwheat seeds. In Current Advances in Buckwheat Research; Skinshu University Press: Matsumoto, Japan, 1995; pp 965-974). It was predicted that FA02 would be cleaved to generate two separate components, a 41.3 kDa alpha -subunit and a 21 kDa beta -subunit. Antiserum was raised against the deduced FA02 beta -subunit, and immunoblotting of total protein from buckwheat seeds (F. esculentum M. and Fagopyrum tartaricum Gaertn.) revealed that several groups of proteins reacted with the antiserum. Polypeptides in the 23-25 kDa range displayed the greatest reactivity.
- リンク情報
- ID情報
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- DOI : 10.1021/jf0011485
- ISSN : 0021-8561
- Web of Science ID : WOS:000168260000026