2016年4月
Screening of dietary antioxidants against mitochondria-mediated oxidative stress by visualization of intracellular redox state
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- ,
- ,
- 巻
- 80
- 号
- 4
- 開始ページ
- 726
- 終了ページ
- 734
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/09168451.2015.1123607
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Mitochondrial impairment and the resulting generation of reactive oxygen species (ROS) have been associated with aging and its related pathological conditions. Recently, dietary antioxidants have gained significant attention as potential preventive and therapeutic agents against ROS-generated aging and pathological conditions. We previously demonstrated that food-derived antioxidants prevented intracellular oxidative stress under proteasome inhibition conditions, which was attributed to mitochondrial dysfunction and ROS generation, followed by cell death. Here, we further screened dietary antioxidants for their activity as redox modulators by visualization of the redox state using Redoxfluor, a fluorescent protein redox probe. Direct alleviation of ROS by antioxidants, but not induction of antioxidative enzymes, prevented mitochondria-mediated intracellular oxidation. The effective antioxidants scavenged mitochondrial ROS and suppressed cell death. Our study indicates that redox visualization under mitochondria-mediated oxidative stress is useful for screening potential antioxidants to counteract mitochondrial dysfunction, which has been implicated in aging and the pathogenesis of aging-related diseases.
- リンク情報
- ID情報
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- DOI : 10.1080/09168451.2015.1123607
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 26967637
- Web of Science ID : WOS:000371994100005