論文

査読有り
2013年3月

A fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae

FEBS LETTERS
  • Masahide Oku
  • ,
  • Jun Hoseki
  • ,
  • Yayoi Ichiki
  • ,
  • Yasuyoshi Sakai

587
6
開始ページ
793
終了ページ
798
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.febslet.2013.02.003
出版者・発行元
ELSEVIER SCIENCE BV

The physiological roles of the thioredoxin isozymes in the yeast Saccharomyces cerevisiae were investigated using a novel FRET-based redox probe, Redoxfluor. After establishing responsiveness of the probe toward thioredoxin, we followed the fluorescence signal of Redoxfluor expressed in the yeast and found that one of the thioredoxin isozymes, Trx2, was required for maintaining the redox status when stationary culture of the organism was exposed to starvation and mild-heat stresses. The failure to maintain redox balance under the tested condition preceded decreased viability of the trx2 mutants, indicating the functional importance of the cytoplasmic thioredoxin in adaptation to environmental changes. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.febslet.2013.02.003
CiNii Articles
http://ci.nii.ac.jp/naid/120005244260
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23416294
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000315947400040&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.febslet.2013.02.003
  • ISSN : 0014-5793
  • eISSN : 1873-3468
  • CiNii Articles ID : 120005244260
  • PubMed ID : 23416294
  • Web of Science ID : WOS:000315947400040

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