2013年3月
A fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae
FEBS LETTERS
- ,
- ,
- ,
- 巻
- 587
- 号
- 6
- 開始ページ
- 793
- 終了ページ
- 798
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.febslet.2013.02.003
- 出版者・発行元
- ELSEVIER SCIENCE BV
The physiological roles of the thioredoxin isozymes in the yeast Saccharomyces cerevisiae were investigated using a novel FRET-based redox probe, Redoxfluor. After establishing responsiveness of the probe toward thioredoxin, we followed the fluorescence signal of Redoxfluor expressed in the yeast and found that one of the thioredoxin isozymes, Trx2, was required for maintaining the redox status when stationary culture of the organism was exposed to starvation and mild-heat stresses. The failure to maintain redox balance under the tested condition preceded decreased viability of the trx2 mutants, indicating the functional importance of the cytoplasmic thioredoxin in adaptation to environmental changes. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.febslet.2013.02.003
- CiNii Articles
- http://ci.nii.ac.jp/naid/120005244260
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/23416294
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000315947400040&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.febslet.2013.02.003
- ISSN : 0014-5793
- eISSN : 1873-3468
- CiNii Articles ID : 120005244260
- PubMed ID : 23416294
- Web of Science ID : WOS:000315947400040