2010年8月
The Peroxisomal Catalase Gene in the Methylotrophic Yeast Pichia methanolica
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 74
- 号
- 8
- 開始ページ
- 1733
- 終了ページ
- 1735
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.100329
- 出版者・発行元
- TAYLOR & FRANCIS LTD
In this paper, we describe the CTA1 gene, which encodes a peroxisomal catalase in the methylotrophic yeast Pichia methanolica. The P. methanolica CTA1 gene (PmCTA1) comprises a 1,530-bp open reading frame corresponding to a protein of 510 amino acid residues, and its deduced amino acid sequence shows high similarity to those of Cta1ps from other methylotrophic yeasts (about 79%). Expression of PinCTA1 in a peroxisomal catalase-depleted (Cbcta1 Delta) Candida boidinii strain restored the methylotrophic growth of the host strain, while the expression of PmCTA1-Delta SRL, which lacks peroxisome targeting signal type 1, did not. In P. methanolica, expression of PmCTA1 was induced when cells were grown on peroxisome-inducing carbon sources, viz., methanol, oleate, and D-alanine. Taken together, these results indicate that PmCTA1 encodes a functional peroxisomal catalase in P. methanolica.
- リンク情報
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- DOI
- https://doi.org/10.1271/bbb.100329
- CiNii Articles
- http://ci.nii.ac.jp/naid/10027558835
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/9143107
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000281722200043&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1271/bbb.100329
- ISSN : 0916-8451
- eISSN : 1347-6947
- CiNii Articles ID : 10027558835
- PubMed ID : 9143107
- Web of Science ID : WOS:000281722200043