2010年9月
Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano
NATURE METHODS
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- 巻
- 7
- 号
- 9
- 開始ページ
- 729
- 終了ページ
- U88
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/nmeth.1488
- 出版者・発行元
- NATURE PUBLISHING GROUP
We report ultrasensitive Ca2+ indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca2+-sensing domain of a genetically encoded Ca2+ indicator, YC2.60 or YC3.60. Their high Ca2+ affinities (K-d = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca2+ transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks.
- リンク情報
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- DOI
- https://doi.org/10.1038/nmeth.1488
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20693999
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000281429200017&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/20693999
- URL
- http://orcid.org/0000-0001-5040-8723
- ID情報
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- DOI : 10.1038/nmeth.1488
- ISSN : 1548-7091
- eISSN : 1548-7105
- ORCIDのPut Code : 12736386
- PubMed ID : 20693999
- Web of Science ID : WOS:000281429200017