論文

査読有り
2017年2月

CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene

SCIENTIFIC REPORTS
  • Michiko Hirose
  • ,
  • Ayumi Hasegawa
  • ,
  • Keiji Mochida
  • ,
  • Shogo Matoba
  • ,
  • Yuki Hatanaka
  • ,
  • Kimiko Inoue
  • ,
  • Tatsuhiko Goto
  • ,
  • Hideki Kaneda
  • ,
  • Ikuko Yamada
  • ,
  • Tamio Furuse
  • ,
  • Kuniya Abe
  • ,
  • Yoshihisa Uenoyama
  • ,
  • Hiroko Tsukamura
  • ,
  • Shigeharu Wakana
  • ,
  • Arata Honda
  • ,
  • Atsuo Ogura

7
開始ページ
42476
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep42476
出版者・発行元
NATURE PUBLISHING GROUP

Wild-derived mice have contributed to experimental mouse genetics by virtue of their genetic diversity, which may help increase the chance of identifying novel modifier genes responsible for specific phenotypes and diseases. However, gene targeting using wild-derived mice has been unsuccessful because of the unavailability of stable embryonic stem cells. Here, we report that CRISPR/Cas9mediated gene targeting can be applied to the Japanese wild-derived MSM/Ms strain (Mus musculus molossinus). We targeted the nonagouti (a) gene encoding the agouti protein that is localized in hair and the brain. We obtained three homozygous knockout mice as founders, all showing black coat colour. While homozygous knockout offspring were physiologically indistinguishable from wild-type littermates, they showed specific domesticated behaviours: hypoactivity in the dark phase and a decline in the avoidance of a human hand. These phenotypes were consistent over subsequent generations. Our findings support the empirical hypothesis that nonagouti is a domestication-linked gene, the loss of which might repress aggressive behaviour.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1038/srep42476
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28195201
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000393942100002&DestApp=WOS_CPL