論文

査読有り
2014年6月

A novel parameter, cell-cycle progression index, for radiation dose absorbed estimation in the premature chromosome condensation assay

RADIATION PROTECTION DOSIMETRY
  • Tomisato Miura
  • ,
  • Akifumi Nakata
  • ,
  • Kosuke Kasai
  • ,
  • Manabu Nakano
  • ,
  • Yu Abe
  • ,
  • Eiki Tsushima
  • ,
  • Natalia I. Ossetrova
  • ,
  • Mitsuaki A. Yoshida
  • ,
  • William F. Blakely

159
1-4
開始ページ
52
終了ページ
60
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/rpd/ncu126
出版者・発行元
OXFORD UNIV PRESS

The calyculin A-induced premature chromosome condensation (PCC) assay is a simple and useful method for assessing the cell-cycle distribution in cells, since calyculin A induces chromosome condensation in various phases of the cell cycle. In this study, a novel parameter, the cell-cycle progression index (CPI), in the PCC assay was validated as a novel biomarker for biodosimetry. Peripheral blood was drawn from healthy donors after informed consent was obtained. CPI was investigated using a human peripheral blood lymphocyte (PBL) ex vivo irradiation (Co-60-gamma rays: similar to 0.6 Gy min(-1), or X ray: 1.0 Gy min(-1); 0-10 Gy) model. The calyculin A-induced PCC assay was performed for chromosome preparation. PCC cells were divided into the following five categories according to cell-cycle stage: non-PCC, G1-PCC, S-PCC, G2/M-PCC and M/A-PCC cells. CPI was calculated as the ratio of G2/M-PCC cells to G1-PCC cells. The PCC-stage distribution varied markedly with irradiation doses. The G1-PCC cell fraction was significantly reduced, and the G2/M-PCC cell fraction increased, in 10-Gy-irradiated PBL after 48 h of culture. CPI levels were fitted to an exponential dose-response curve with gamma-ray irradiation [y = 0.6729 + 0.3934 exp(0.5685D), r = 1.0000, p < 0.0001] and X-ray irradiation [y = -0.3743 + 0.9744 exp(0.3321D), r = 0.9999, p < 0.0001]. There were no significant individual (p = 0.853) or gender effects (p = 0.951) on the CPI in the human peripheral blood ex vivo irradiation model. Furthermore, CPI measurements are rapid (< 15 min per case). These results suggest that the CPI is a useful screening tool for the assessment of radiation doses received ranging from 0 to 10 Gy in radiation exposure early after a radiation event, especially after a mass-casualty radiological incident.

リンク情報
DOI
https://doi.org/10.1093/rpd/ncu126
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24743756
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000338829100009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/rpd/ncu126
  • ISSN : 0144-8420
  • eISSN : 1742-3406
  • PubMed ID : 24743756
  • Web of Science ID : WOS:000338829100009

エクスポート
BibTeX RIS