論文

査読有り
2017年1月

Fucosyltransferases produce N-glycans containing core L-galactose

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Hiroyuki Ohashi
  • ,
  • Takao Ohashi
  • ,
  • Hiroyuki Kajiura
  • ,
  • Ryo Misaki
  • ,
  • Shinichi Kitamura
  • ,
  • Kazuhito Fujiyama

483
1
開始ページ
658
終了ページ
663
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2016.12.087
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

L-Galactose (L-Gal) containing N-glycans and cell wall polysaccharides have been detected in the L-Fuc deficient mur1 mutant of Arabidopsis thaliana. The L-Gal residue is thought to be transferred from GDP-L-Gal, which is a structurally related analog of GDP-L-Fuc, but in vitro L-galactosylation activity has never been detected. In this study, we carried out preparative scale GDP-L-Gal synthesis using recombinant A. thaliana GDP-mannose-3',5'-epimerase. We also demonstrated the L-galactosylation assay of mouse alpha 1,6-fucosyltransferase (MmFUT8) and A. thaliana a1,3-fucosyltransferase (AtFucTA). Both fucosyltransferases showed L-galactosylation activity from GDP-L-Gal to asparagine-linked N-acetyl-beta-D-glucosamine of asialo-agalacto-bi-antennary N-glycan instead of L-fucosylation. In addition, the apparent Km values of MmFUT8 and AtFucTA suggest that L-Fuc was preferentially transferred to N-glycan compared with L-Gal by fucosyltransferases. Our results clearly demonstrate that MmFUT8 and AtFucTA transfer L-Gal residues from GDP-L-Gal and synthesize L-Gal containing N-glycan in vitro. (C) 2016 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2016.12.087
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27993676
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397259000102&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2016.12.087
  • ISSN : 0006-291X
  • eISSN : 1090-2104
  • PubMed ID : 27993676
  • Web of Science ID : WOS:000397259000102

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