論文

査読有り
2017年2月

The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • Shoichi Wakitani
  • ,
  • Daigo Yokoi
  • ,
  • Yuichi Hidaka
  • ,
  • Koichiro Nishino

79
2
開始ページ
230
終了ページ
237
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1292/jvms.16-0321
出版者・発行元
JAPAN SOC VET SCI

Runt-related transcription factor 2 (Runx2) is essential for osteogenesis. This study aimes at identification of the genomic region differentially methylated in DNA for regulation of Runx2 expression. In the proximal promoter of mouse Runx2, DNA methylation was frequent at the region further than 3 kb relative to the transcription start site, in contrast to lower methylation status of the closer locus within 2 kb from the transcription start site. At the intermediate part, we identified a novel differentially methylated region in the Runx2 promoter region (Runx2-DMR): from -2.7 to -2.2 kb relative to the start site of Runx2 transcription in mice. In this region, the DNA methylation rate correlated negatively with Runx2 expression among mouse organs as well as among primary cultures of bone marrow from different dogs. Induction of mouse and dog mesenchymal-like cells into osteoblastic differentiation decreased the methylation rate of Runx2DMR. Thus, in this study, we identified a novel genomic region in which DNA methylation status is related to Runx2 expression and detected demethylation of Runx2-DMR during osteoblastic differentiation in mouse and dog.

リンク情報
DOI
https://doi.org/10.1292/jvms.16-0321
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27916785
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397703200001&DestApp=WOS_CPL
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85011587124&origin=inward
ID情報
  • DOI : 10.1292/jvms.16-0321
  • ISSN : 0916-7250
  • eISSN : 1347-7439
  • PubMed ID : 27916785
  • Web of Science ID : WOS:000397703200001

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