論文

査読有り
2017年8月

Higher Arc Nucleus-to-Cytoplasm Ratio during Sleep in the Superficial Layers of the Mouse Cortex

FRONTIERS IN NEURAL CIRCUITS
  • Sakiko Honjoh
  • ,
  • Luisa de Vivo
  • ,
  • Hiroyuki Okuno
  • ,
  • Haruhiko Bito
  • ,
  • Giulio Tononi
  • ,
  • Chiara Cirelli

11
開始ページ
60
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3389/fncir.2017.00060
出版者・発行元
FRONTIERS MEDIA SA

The activity-regulated cytoskeleton associated protein Arc is strongly and quickly upregulated by neuronal activity, synaptic potentiation and learning. Arc entry in the synapse is followed by the endocytosis of glutamatergic AMPA receptors (AMPARs), and its nuclear accumulation has been shown in vitro to result in a small decline in the transcription of the GluA1 subunit of AMPARs. Since these effects result in a decline in synaptic strength, we asked whether a change in Arc dynamics may temporally correlate with sleep-dependent GluA1 down-regulation. We measured the ratio of nuclear to cytoplasmic Arc expression (Arc Nuc/Cyto) in the cerebral cortex of EGFP-Arc transgenic mice that were awake most of the night and then perfused immediately before lights on (W mice), or were awake most of the night and then allowed to sleep (S mice) or sleep deprived (SD mice) for the first 2 h of the light phase. In primary motor cortex (M1), neurons with high levels of nuclear Arc (High Arc cells) were present in all mice, but in these cells Arc Nuc/Cyto was higher in S mice than in W mice and, importantly, similar to 15% higher in S mice than in SD mice collected at the same time of day, ruling out circadian effects. Greater Arc Nuc/Cyto with sleep was observed in the superficial layers of M1, but not in the deep layers. In High Arc cells, Arc Nuc/Cyto was also similar to 15%-30% higher in S mice than in W and SD mice in the superficial layers of primary somatosensory cortex (S1) and cingulate cortex area 1 (Cg1). In High Arc Cells of Cg1, Arc Nuc/Cyto and cytoplasmic levels of GluA1 immunoreactivities in the soma were also negatively correlated, independent of behavioral state. Thus, Arc moves to the nucleus during both sleep and wake, but its nuclear to cytoplasmic ratio increases with sleep in the superficial layers of several cortical areas. It remains to be determined whether the relative increase in nuclear Arc contributes significantly to the overall decline in the strength of excitatory synapses that occurs during sleep. Similarly, it remains to be determined whether the entry of Arc into specific synapses is gated by sleep.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.3389/fncir.2017.00060
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28878629
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000409010300001&DestApp=WOS_CPL
ID情報
  • DOI : 10.3389/fncir.2017.00060
  • ISSN : 1662-5110
  • PubMed ID : 28878629
  • Web of Science ID : WOS:000409010300001

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