論文

査読有り
2004年4月

ERK activation propagates in epithelial cell sheets and regulates their migration during wound healing

CURRENT BIOLOGY
  • Y Matsubayashi
  • ,
  • M Ebisuya
  • ,
  • S Honjoh
  • ,
  • E Nishida

14
8
開始ページ
731
終了ページ
735
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.cub.2004.03.060
出版者・発行元
CELL PRESS

in epithelial cell movements, which occur during wound healing or embryonic morphogenesis, sheets of cells move together as a unit [1, 2]. Molecular mechanisms that regulate this sheet movement have been largely unknown, although cell locomotion or movement mechanisms for individual cells, such as for fibroblastic cells, have been extensively studied [3-5]. Here, we show that, during wound healing, sheets of MDCK epithelial cells migrate coordinately as a unit, and wound-induced activation of ERK MAP kinase (ERK1/2) propagates in cell sheets in accordance with the cell sheet movement. Inhibition of ERK1/2 activation by specific MEK inhibitors or by expressing dominant-negative ERK2 results in marked inhibition of the sheet movement during wound healing, and inhibition of the cell sheet movement by disrupting actin cytoskeleton suppresses propagation of ERK1/2 activation. These results indicate that cell movement and ERK1/2 activation form a positive feedback loop, which facilitates cell sheet migration. Moreover, we find that Src family kinase inhibitors suppress both cell migration and propagation of ERK1/2 activation, suggesting that Src family kinase may participate in this feedback loop. Interestingly, neither cell sheet migration as a unit nor migration-dependent propagation of ERK1/2 activation occurs during wound healing in fibroblastic 3Y1 cells. Thus, our results identify specific requirements of ERK1/2 MAP kinase for epithelial cell sheet movement.

Web of Science ® 被引用回数 : 176

リンク情報
DOI
https://doi.org/10.1016/j.cub.2004.03.060
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15084290
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000220989700027&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.cub.2004.03.060
  • ISSN : 0960-9822
  • PubMed ID : 15084290
  • Web of Science ID : WOS:000220989700027

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