論文

国際誌
2012年4月20日

Dicer has a crucial role in the early stage of adipocyte differentiation, but not in lipid synthesis, in 3T3-L1 cells.

Biochemical and biophysical research communications
  • Yuri Fujimoto
  • ,
  • Yoshimi Nakagawa
  • ,
  • Akiko Shingyouchi
  • ,
  • Naoko Tokushige
  • ,
  • Noriko Nakanishi
  • ,
  • Aoi Satoh
  • ,
  • Takashi Matsuzaka
  • ,
  • Kiyo-aki Ishii
  • ,
  • Hitoshi Iwasaki
  • ,
  • Kazuto Kobayashi
  • ,
  • Shigeru Yatoh
  • ,
  • Hiroaki Suzuki
  • ,
  • Naoya Yahagi
  • ,
  • Osamu Urayama
  • ,
  • Nobuhiro Yamada
  • ,
  • Hitoshi Shimano

420
4
開始ページ
931
終了ページ
6
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2012.03.110

Dicer is a rate-limiting enzyme for microRNA (miRNA) synthesis. To determine the effects of Dicer on adipogenesis, we performed stage-specific knockdown of Dicer using adenovirus encoding short-hairpin RNAi against Dicer in 3T3-L1 cells. When cells were infected with the adenovirus before induction of adipocyte differentiation, Dicer RNAi suppressed the gene expression of inducers of adipocyte differentiation such as PPARγ, C/EBPα, and FAS in 3T3-L1 cells during adipocyte differentiation. Concurrently, both adipocyte differentiation and cellular lipid accumulation were cancelled by Dicer RNAi when compared with control RNAi. Meanwhile, we addressed the roles of Dicer in lipid synthesis and accumulation in the final stages of differentiation. When the differentiated cells at day 4 after induction of differentiation were infected with adenovirus Dicer RNAi, cellular lipid accumulation was unchanged. Consistent with this, Dicer RNAi had no effects on the expression of genes related to cellular lipid accumulation, including PPARγ and FAS. Thus, Dicer controls proadipogenic genes such as C/EBPα and PPARγ in the early, but not in the late, stage of adipogenesis via regulation of miRNA synthesis.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2012.03.110
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22475483
ID情報
  • DOI : 10.1016/j.bbrc.2012.03.110
  • PubMed ID : 22475483

エクスポート
BibTeX RIS