The lower critical field of type-II organic layered superconductor λ-(BETS)2GaCl4 has been estimated by magnetic measurements. The demagnetization factor of a needle-like sample shape has been taken into account for the measurements by applying external field, parallel and perpendicular to the conducting plane. The lower critical field for the field parallel to the conducting plane is 5.2(4) G, in contrast with the previous studies. This allows us to discuss the anisotropy of lower critical field Hc1 in a quasi-two-dimensional organic layered superconductor λ-(BETS)2GaCl4.