Papers

Peer-reviewed
Dec, 2012

Identification of L-3-hydroxykynurenine O-sulfate in the buccal gland secretion of the parasitic lamprey, Lethenteron japonicum

AMINO ACIDS
  • Shoji Odani
  • ,
  • Naoko Ito
  • ,
  • Mai Hasegawa
  • ,
  • Toshio Uchiumi
  • ,
  • Sumihiro Hase

Volume
43
Number
6
First page
2505
Last page
2512
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1007/s00726-012-1331-x
Publisher
SPRINGER WIEN

Parasitic lampreys are known to secrete proteins having anticoagulant and vasodilator activities from the buccal glands during feeding on their host's blood. However, small molecules in the secretion have never been explored in detail. We examined the secretion of Japanese liver lamprey (Lethenteron japonicum) for small molecules and found an intensely fluorescent substance upon gel filtration. After purification by anion-exchange chromatography and reversed-phase HPLC, structure of the compound was determined to be l-3-hydroxykynurenine O-sulfate by NMR- and UV-spectrometry, complemented with enzymatic and chemical degradation. In vertebrates, the sulfate ester of 3-hydroxykynurenine is a compound that has been regarded as a urinary metabolite of tryptophan but not reported from normal tissues to date. Although the function of this molecule in the buccal glands remains to be elucidated, it is remarkable that the same substance was described in 1960s from two species of blood-sucking insects, Rhodnius prolixus and Triatoma infestans, suggesting its potential role in blood-feeding.

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Link information
DOI
https://doi.org/10.1007/s00726-012-1331-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22648634
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000310885500024&DestApp=WOS_CPL
ID information
  • DOI : 10.1007/s00726-012-1331-x
  • ISSN : 0939-4451
  • Pubmed ID : 22648634
  • Web of Science ID : WOS:000310885500024

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