論文

査読有り
2017年5月

Functional Thyroid Follicular cells Differentiation from human-induced Pluripotent stem cells in suspension culture

FRONTIERS IN ENDOCRINOLOGY
  • Ayumi Arauchi
  • ,
  • Katsuhisa Matsuura
  • ,
  • Tatsuya Shimizu
  • ,
  • Teruo Okano

8
開始ページ
103
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3389/fendo.2017.00103
出版者・発行元
FRONTIERS MEDIA SA

The replacement of regenerated thyroid follicular cells (TFCs) is a promising therapeutic strategy for patients with hypothyroidism. Here, we have succeeded in inducing functional TFCs from human-induced pluripotent stem cells (iPSCs) in scalable suspension culture. Differentiation of iPSCs with Activin A treatment produced Sox17- and FoxA2-expressing definitive endodermal cells that also expressed thyroid transcription factors Pax8 and Nkx2-1. Further treatment with thyroid-stimulating hormone (TSH) induced TFCs expressing various types of thyroid proteins including TSH receptor, sodium-iodide symporter, thyroglobulin, and thyroid peroxidase. Interestingly, differentiated cells secreted free thyroxine in vitro. These results indicate successful differentiation of human iPSCs to functional TFCs that may enable us to fabricate thyroid tissues for regenerative medicine and disease models.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.3389/fendo.2017.00103
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28588551
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000402194700002&DestApp=WOS_CPL

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