論文

査読有り
2016年10月

Transplantation of cancerous cell sheets effectively generates tumour-bearing model mice

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
  • Jun Akimoto
  • ,
  • Soichi Takagi
  • ,
  • Masamichi Nakayama
  • ,
  • Ayumi Arauchi
  • ,
  • Masayuki Yamato
  • ,
  • Teruo Okano

10
10
開始ページ
E510
終了ページ
E517
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/term.1850
出版者・発行元
WILEY-BLACKWELL

Tumour-bearing micewere created by transplanting cancerous cell sheets onto the subcutaneous tissue of the dorsal region, using luciferase gene-transfected mammary gland adenocarcinoma cells, 4T1-luc2, to investigate the tumourigenicity of the cell sheet relative to a conventional injection of cell suspension. Contiguous breast cancerous cell sheets were harvested from temperature-responsive culture dishes by reducing the temperature from 37 degrees C to 20 degrees C; the sheets were then transplanted onto the dorsal side of themouse subcutaneous tissue, using a chitin-based supporting membrane. Cell suspensions obtained by trypsin digestion were subcutaneously injected into the dorsal region of mice. The tumour growth of the transplanted cancer cells was evaluated by the tumour volume and by the bioluminescence from luciferase-gene transfected cancer cells, using an in vivo imaging system. The cell sheetmethod improved the 4 T1-luc2 engraftment efficiency in living mouse tissues at the initial stage by 13-fold compared with that from injecting cell suspensions. On day 14 after the transplantation, the tumour formation at the transplanted area of cell sheet-transplanted mice also accelerated, and the mean tumour volume became 1116 mm(3), which was 10 times larger than that in cell suspension-transplanted mice. The cell sheets engrafted on the recipient tissues efficiently due to the preserved extracellularmatrix on their basal sides, such that cancer cells were supplied with sufficient oxygen and nutrients from the host tissues to develop tumour tissues. Therefore, cancerous cell sheet-based transplantation is a promising method for efficiently creating cancer-bearing mice. Copyright (C) 2013 John Wiley & Sons, Ltd.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1002/term.1850
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24668943
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000387786200027&DestApp=WOS_CPL

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