論文

査読有り
2014年11月

Facile cell sheet manipulation and transplantation by using in situ gelation method

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
  • Jun Akimoto
  • ,
  • Ayumi Arauchi
  • ,
  • Masamichi Nakayama
  • ,
  • Ryo Kanaya
  • ,
  • Yuko Iwase
  • ,
  • Soichi Takagi
  • ,
  • Masayuki Yamato
  • ,
  • Teruo Okano

102
8
開始ページ
1659
終了ページ
1668
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/jbm.b.33148
出版者・発行元
WILEY

Cell sheets harvested from temperature-responsive cell culture dishes (TRDs) has attracted considerable attention as effective tools for reconstructing the lost functions of tissues and organs in the regenerative medicine field. However, because of their thinness, handling problems sometimes arise when transferring cell sheets from a TRD to a target surface. In this study, we developed a facile cell transfer method referred to as in situ gelation by using both gelatin hydrogel and a support membrane. Gelation and low-temperature processes were simultaneously performed on TRD. Confluent cultured cells were efficiently harvested from TRD in less than 5 min by decreasing the incubation temperature to 20 degrees C. Harvested cells were found to maintain their cell viability, extracellular matrix, and original shape, thus allowing transfer of the cells to another surface with a short incubation time at 37 degrees C. This method is applicable for various cell types regardless of the formation of tight cell-cell junctions. In addition, because of the high flexibility of the gelatin-coated membrane, cells were efficiently transferred to the surface of a mouse subcutis and liver. When compared with conventional cell sheet manipulation methods, the interaction between the cell surface and membrane was reinforced by the uniformly formed gelatin gel layer without using a special device. Therefore, the in situ gelation method is a promising technique for cell sheet-based tissue engineering and regenerative medicine. (C) 2014 Wiley Periodicals, Inc.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1002/jbm.b.33148
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24665017
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000343011400007&DestApp=WOS_CPL

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