論文

査読有り
2016年10月

Diaphanous gene mutation affects spiral cleavage and chirality in snails

SCIENTIFIC REPORTS
  • Reiko Kuroda
  • ,
  • Kohei Fujikura
  • ,
  • Masanori Abe
  • ,
  • Yuji Hosoiri
  • ,
  • Shuichi Asakawa
  • ,
  • Miho Shimizu
  • ,
  • Shin Umeda
  • ,
  • Futaba Ichikawa
  • ,
  • Hiromi Takahashi

6
開始ページ
34809
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep34809
出版者・発行元
NATURE PUBLISHING GROUP

L-R (left and right) symmetry breaking during embryogenesis and the establishment of asymmetric body plan are key issues in developmental biology, but the onset including the handedness-determining gene locus still remains unknown. Using pure dextral (DD) and sinistral (dd) strains of the pond snail Lymnaea stagnalis as well as its F-2 through to F-10 backcrossed lines, the single handedness-determininggene locus was mapped by genetic linkage analysis, BAC cloning and chromosome walking. We have identified the actin-related diaphanous gene Lsdia1 as the strongest candidate. Although the cDNA and derived amino acid sequences of the tandemly duplicated Lsdia1 and Lsdia2 genes are very similar, we could discriminate the two genes/proteins in our molecular biology experiments. The Lsdia1 gene of the sinistral strain carries a frameshift mutation that abrogates full-length LsDia1 protein expression. In the dextral strain, it is already translated prior to oviposition. Expression of Lsdia1 (only in the dextral strain) and Lsdia2 (in both chirality) decreases after the 1-cell stage, with no asymmetric localization throughout. The evolutionary relationships among body handedness, SD/SI (spiral deformation/spindle inclination) at the third cleavage, and expression of diaphanous proteins are discussed in comparison with three other pond snails (L. peregra, Physa acuta and Indoplanorbis exustus).

Web of Science ® 被引用回数 : 21

リンク情報
DOI
https://doi.org/10.1038/srep34809
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27708420
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000384766500001&DestApp=WOS_CPL

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