論文

査読有り
2014年

Spiral cleavages determine the left-right body plan by regulating Nodal pathway in monomorphic gastropods, Physa acuta

INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
  • Masanori Abe
  • ,
  • Hiromi Takahashi
  • ,
  • Reiko Kuroda

58
6-8
開始ページ
513
終了ページ
520
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1387/ijdb.140087rk
出版者・発行元
U B C PRESS

The handedness of gastropods is genetically determined, but the molecular nature of the gene responsible and the associated mechanisms remain unknown. In order to characterize the chiromorphogenesis pathway starting from the gene to the left-right asymmetric body plan, we have closely analyzed the cytoskeletal dynamics of the Physa (P.) acuta embryo, a fresh water non-dimorphic sinistral snail, during the early developmental stage by mechanically altering the handedness of the embryos at the critical spiral third cleavage. A fertile situs in versus was created and the nodal-Pitx gene expression patterns were completely mirror imaged to the wild type at the trochophore stage.Together with our previous work on Lymnaea (L.) stagnalis, we could show that chirality is established at the third cleavage, as dictated by the single handedness-determining gene locus, and then chirality information is transferred via subsequent spiral fourth and fifth cleavages to the later developmental stage, dictating the nodal-Pitx expression pathway. The cytoskeletal dynamics of manipulated and non-manipulated embryos of sinistral P acute and dextral dominant L. stagnalis are compared.

Web of Science ® 被引用回数 : 12

リンク情報
DOI
https://doi.org/10.1387/ijdb.140087rk
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25690966
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000348552200011&DestApp=WOS_CPL

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