論文

査読有り
2014年8月

Inhibition of p16 tumor suppressor gene expression via promoter hypermethylation in canine lymphoid tumor cells

RESEARCH IN VETERINARY SCIENCE
  • Aki Fujiwara-Igarashi
  • ,
  • Yuko Goto-Koshino
  • ,
  • Hiroyuki Mochizuki
  • ,
  • Masahiko Sato
  • ,
  • Yasuhito Fujino
  • ,
  • Koichi Ohno
  • ,
  • Hajime Tsujimoto

97
1
開始ページ
60
終了ページ
63
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.rvsc.2014.04.008
出版者・発行元
ELSEVIER SCI LTD

To investigate the epigenetic regulation of the p16 gene in canine lymphoid tumor cells, its methylation status was examined in four canine lymphoid tumor cell lines. In three canine lymphoid tumor cell lines (CLBL-1, GL-1, and UL-1) with low-level p16 mRNA expression, 20 CpG sites in the promoter region of p16 gene were consistently methylated although all of the CpG sites were not methylated in another cell line (CL-1) and normal lymph node cells. The expression level of p16 mRNA in these three cell lines was restored after cultivation in the presence of a methylation inhibitor, 5-Aza-2'-deoxycitidine, indicating inactivation of p16 gene via hypermethylation. This study revealed the inactivation of p16 gene through hypermethylation of its CpG island in a fraction of canine lymphoid tumor cells. (C) 2014 Elsevier Ltd. All rights reserved.


リンク情報
DOI
https://doi.org/10.1016/j.rvsc.2014.04.008
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24815345
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000340852600011&DestApp=WOS_CPL