論文

国際誌
2023年10月29日

Tumor-suppressive microRNA-152 inhibits the proliferation of Ewing's sarcoma cells by targeting CDK5R1.

Scientific reports
  • Masanori Kawano
  • ,
  • Kazuhiro Tanaka
  • ,
  • Ichiro Itonaga
  • ,
  • Tatsuya Iwasaki
  • ,
  • Yuta Kubota
  • ,
  • Hiroshi Tsumura

13
1
開始ページ
18546
終了ページ
18546
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-023-45833-6

We elucidated the mechanism through which the reduced expression of miR-152 leads to the overexpression of its target cyclin-dependent kinase-5 activator 1 (CDK5R1) in Ewing's sarcoma (ES) cells and the role of this mechanism in the proliferation of ES cells. To explore possible oncogenic factors in ES, we conducted microarray-based investigation and profiled the changes in miRNA expression and their effects on downstream mRNAs in five ES cell lines and human mesenchymal stem cells (hMSCs). miR-152 was significantly downregulated, while cyclin-dependent kinase-5 activator 1 (CDK5R1) expression was significantly upregulated in all tested ES cells as compared to hMSCs. The overexpression of CDK5R1 led to the activation of CDK5, enabling the phosphorylation of retinoblastoma protein and persistent overexpression of CCNE. Moreover, miR-152 suppressed cell proliferation via cell cycle retardation, and its upregulation reduced tumor size and CCNE expression in tumor tissues. The overexpression of cyclin E (CCNE) has been detected in ES cells, but the detailed mechanisms have not been previously elucidated. These findings identify the miR152-CDK5R1 signaling axis as a critical mechanism for tumorigenesis that may serve as a new therapeutic target in Ewing's sarcoma. We believe that our results will aid in the development of effective treatment strategies for patients with ES.

リンク情報
DOI
https://doi.org/10.1038/s41598-023-45833-6
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/37899376
ID情報
  • DOI : 10.1038/s41598-023-45833-6
  • PubMed ID : 37899376

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