2009年9月
Generation of Cortactin Floxed Mice and Cellular Analysis of Motility in Fibroblasts
GENESIS
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- 巻
- 47
- 号
- 9
- 開始ページ
- 638
- 終了ページ
- 646
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/dvg.20544
- 出版者・発行元
- WILEY-LISS
Cortactin is an F-actin binding protein that has been suggested to play key roles in various cellular functions. Here, we generated mice carrying floxed alleles of the cortactin (Cttn) gene (Cttn(flox/flox) mice). Expression of Cre recombinase in mouse embryonic fibroblasts (MEFs) isolated from Cttn(flox/flox) embryos depleted cortactin within days, without disturbing F-actin distribution and localization of multiple actin-binding proteins. Cre-mediated deletion of Cttn also did not affect cell migration. To obtain mice with a Cttn null allele, we next crossed Cttn(flox/flox) mice with transgenic mice that express Cre recombinase ubiquitously. Western blot and immunocytochemical analysis confirmed complete elimination of cortactin expression in MEFs carrying homozygously Cttn null alleles. However, we found no marked alteration of F-actin organization and cell migration in Cttn null-MEFs. Thus, our results indicate that depletion of cortactin in MEFs does not profoundly influence actin-dependent cell motility. genesis 47:638-646, 2009. (C) 2009 Wiley-Liss, Inc.
- リンク情報
- ID情報
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- DOI : 10.1002/dvg.20544
- ISSN : 1526-954X
- PubMed ID : 19621352
- Web of Science ID : WOS:000270029900008