論文

査読有り
2018年

Generation of transgenic mouse line with prostate-specific expression of codon-improved Cre recombinase

Prostate International
  • Mayuko Kanayama
  • ,
  • Kazuki Nakao
  • ,
  • Shigeo Horie
  • ,
  • Atsu Aiba

6
3
開始ページ
99—103
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.prnil.2018.04.003
出版者・発行元
Elsevier B.V.

Background: Genetically engineered mouse models are useful tools to decipher molecular mechanisms of diseases. As for prostates, a rat probasin promoter has been widely used to drive prostate-specific gene expression. To optimize its codon usage to that of mammals, we used codon-improved Cre recombinase (iCre) for prostate-specific Cre-loxP recombination. Materials and methods: We generated transgenic mice that express iCre driven by conventional probasin promoter in a prostate-specific manner (PB-iCre). Linearized PB-iCre transgene deoxyribonucleic acids (DNAs) were microinjected into pronuclei of fertilized mouse embryos. The integration of the transgene was confirmed by Southern blot analysis. A line of transgenic mice expressing a sufficient amount of iCre mRNA in its prostate was selected. To test recombinase activity of PB-iCre in vivo, its offspring was crossbred with Ptenflox/flox mice in which murine prostate adenocarcinoma is reported to occur upon excision of loxP-flanked regions. Results: Eight founder animals were obtained, all of which showed germ line integration of PB-iCre transgene by Southern blot analysis. Among them, the prostate from only one line (line 58) expressed a sufficient amount of iCre mRNA. This line was crossbred with Ptenflox/flox mice to generate PB-iCre58/Ptenflox/flox. As a result, 12-week-old PB-iCre58/Ptenflox/flox mice presented with prostate adenocarcinoma that was histologically similar to human cribriform prostate cancer of Gleason grade 4. Conclusions: We have successfully established a transgenic mouse line that expresses iCre in a prostate-specific manner.

リンク情報
DOI
https://doi.org/10.1016/j.prnil.2018.04.003
URL
http://europepmc.org/articles/PMC6104291
URL
http://orcid.org/0000-0002-8192-0778
ID情報
  • DOI : 10.1016/j.prnil.2018.04.003
  • ISSN : 2287-903X
  • ISSN : 2287-8882
  • ORCIDのPut Code : 49323127
  • SCOPUS ID : 85047076029

エクスポート
BibTeX RIS