論文

査読有り
2016年9月

CRMP1 and CRMP2 have synergistic but distinct roles in dendritic development

GENES TO CELLS
  • Hiroko Makihara
  • ,
  • Shiori Nakai
  • ,
  • Wataru Ohkubo
  • ,
  • Naoya Yamashita
  • ,
  • Fumio Nakamura
  • ,
  • Hiroshi Kiyonari
  • ,
  • Go Shioi
  • ,
  • Aoi Jitsuki-Takahashi
  • ,
  • Haruko Nakamura
  • ,
  • Fumiaki Tanaka
  • ,
  • Tomoko Akase
  • ,
  • Pappachan Kolattukudy
  • ,
  • Yoshio Goshima

21
9
開始ページ
994
終了ページ
1005
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/gtc.12399
出版者・発行元
WILEY-BLACKWELL

Collapsin response mediator protein 2, CRMP2, has been identified as an intracellular signaling mediator for Semaphorin 3A (Sema3A). CRMP2 plays a key role in axon guidance, dendritic morphogenesis, and cell polarization. It has been also implicated in a variety of neurological and psychiatric disorders. However, the invivo functions of CRMP2 remain unknown. We generated CRMP2 gene-deficient (crmp2(-/-)) mice. The crmp2(-/-) mice showed irregular development of dendritic spines in cortical neurons. The density of dendritic spines was reduced in the cortical layer V pyramidal neurons of crmp2(-/-) mice as well as in those of sema3A(-/-) and crmp1(-/-) mice. However, no abnormality was found in dendritic patterning in crmp2(-/-) compared to wild-type (WT) neurons. The level of CRMP1 was increased in crmp2(-/-), but the level of CRMP2 was not altered in crmp1(-/-) compared to WT cortical brain lysates. Dendritic spine density and branching were reduced in double-heterozygous sema3A(+/-);crmp2(+/-) and sema3A(+/-);crmp1(+/-) mice. The phenotypic defects had no genetic interaction between crmp1 and crmp2. These findings suggest that both CRMP1 and CRMP2 mediate Sema3A signaling to regulate dendritic spine maturation and patterning, but through overlapping and distinct signaling pathways.

Web of Science ® 被引用回数 : 36

リンク情報
DOI
https://doi.org/10.1111/gtc.12399
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27480924
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000384089100005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/gtc.12399
  • ISSN : 1356-9597
  • eISSN : 1365-2443
  • PubMed ID : 27480924
  • Web of Science ID : WOS:000384089100005

エクスポート
BibTeX RIS