2015年9月
Anti-Semaphorin 3A neutralization monoclonal antibody prevents sepsis development in lipopolysaccharide-treated mice
INTERNATIONAL IMMUNOLOGY
- 巻
- 27
- 号
- 9
- 開始ページ
- 459
- 終了ページ
- 466
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/intimm/dxv014
- 出版者・発行元
- OXFORD UNIV PRESS
A novel anti-semaphorin antibody that ameliorates sepsis.Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A both in vitro and in vivo. The anti-Sema3A neutralization chick IgM antibodies were screened by combining an autonomously diversifying library selection system and an in vitro growth cone collapse assay. We further developed function-blocking chick-mouse chimeric and humanized anti-Sema3A antibodies. We found that our anti-Sema3A antibodies were effective for improving the survival rate in lipopolysaccharide-induced sepsis in mice. Our antibody is a potential therapeutic agent that may prevent the onset of or alleviate symptoms of human diseases associated with Sema3A.
- リンク情報
- ID情報
-
- DOI : 10.1093/intimm/dxv014
- ISSN : 0953-8178
- eISSN : 1460-2377
- PubMed ID : 25855660
- Web of Science ID : WOS:000361220800005