論文

査読有り 筆頭著者
2015年9月

Anti-Semaphorin 3A neutralization monoclonal antibody prevents sepsis development in lipopolysaccharide-treated mice

INTERNATIONAL IMMUNOLOGY
  • *Naoya Yamashita
  • ,
  • *Aoi Jitsuki-Takahashi
  • ,
  • Miyuki Ogawara
  • ,
  • Wataru Ohkubo
  • ,
  • Tomomi Araki
  • ,
  • Chie Hotta
  • ,
  • Tomohiko Tamura
  • ,
  • Shu-ichi Hashimoto
  • ,
  • Takashi Yabuki
  • ,
  • Toru Tsuji
  • ,
  • Yukie Sasakura
  • ,
  • Hiromi Okumura
  • ,
  • Aki Takaiwa
  • ,
  • Chika Koyama
  • ,
  • Koji Murakami
  • ,
  • Yoshio Goshima

27
9
開始ページ
459
終了ページ
466
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/intimm/dxv014
出版者・発行元
OXFORD UNIV PRESS

A novel anti-semaphorin antibody that ameliorates sepsis.Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A both in vitro and in vivo. The anti-Sema3A neutralization chick IgM antibodies were screened by combining an autonomously diversifying library selection system and an in vitro growth cone collapse assay. We further developed function-blocking chick-mouse chimeric and humanized anti-Sema3A antibodies. We found that our anti-Sema3A antibodies were effective for improving the survival rate in lipopolysaccharide-induced sepsis in mice. Our antibody is a potential therapeutic agent that may prevent the onset of or alleviate symptoms of human diseases associated with Sema3A.

Web of Science ® 被引用回数 : 18

リンク情報
DOI
https://doi.org/10.1093/intimm/dxv014
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25855660
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000361220800005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/intimm/dxv014
  • ISSN : 0953-8178
  • eISSN : 1460-2377
  • PubMed ID : 25855660
  • Web of Science ID : WOS:000361220800005

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