The aim of this study was to evaluate epigenetics small molecule effect on acceleration of bone formation through regulation of inflammation, cell migration, and osteoblast differentiation. This study would help to establish bone augmentation therapy with multiple approach such as increasing of cell recruitment, vascularization, and mineralization. Valproic acid which had HDACi effect was used as epigenetic small molecule. VPA treatment with osteogenic differentiation stimulation showed significant increase in expressions of osteogenic marker gene compare to the osteogenic differentiation stimulation alone. VPA treatment also increased cell recruitment related gene and vascularization related gene expressions.
VPA treated cell transplantation to the calvarias cavity showed more bone formation than control cell transplantation.