2017年10月
Engineering a short-chain dehydrogenase/reductase for the stereoselective production of (2S, 3R, 4S)-4-hydroxyisoleucine with three asymmetric centers
SCIENTIFIC REPORTS
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- 巻
- 7
- 号
- 開始ページ
- 13703
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41598-017-13978-w
- 出版者・発行元
- NATURE PUBLISHING GROUP
Fenugreek is a dietary supplement for anti-aging and human health. (2S, 3R, 4S)-4-hydroxyisoleucine (4-HIL), which is extracted from fenugreek seeds, is expected to be a promising orally active drug for diabetes and diabetic nephropathy because of its insulinotropic effect. Although several chemical synthesis methods of 4-HIL have been proposed, these methods require multistep reactions to control the stereochemistry of 4-HIL. In this study, we modified the key enzyme 4-HIL dehydrogenase (HILDH) to overcome the biggest limitation in commercial-scale production of 4-HIL. As a result, an effective one-step carbonyl reduction to produce (2S, 3R, 4S)-4-HIL was successfully accomplished with strict stereoselectivity (>99% de). Mass production of (2S, 3R, 4S)-4-HIL by our synthetic method could have a significant contribution to the prevention of diabetes, dyslipidemia, and Alzheimer's disease. (120 words/200 words)
- リンク情報
- ID情報
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- DOI : 10.1038/s41598-017-13978-w
- ISSN : 2045-2322
- Web of Science ID : WOS:000413357500069