2015年12月
Attenuation of autophagic-proteolysis in C2C12 cells by saccharopine
MOLECULAR AND CELLULAR BIOCHEMISTRY
- ,
- ,
- 巻
- 410
- 号
- 1-2
- 開始ページ
- 93
- 終了ページ
- 100
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s11010-015-2541-9
- 出版者・発行元
- SPRINGER
Muscle wasting impairs physical function and leads people to a bedridden state. We previously demonstrated that lysine (Lys) suppresses autophagic-proteolysis through the Akt pathway. However, the effect of metabolites of Lys on proteolysis is unclear. In this study, we investigated the effect of saccharopine (Sac), a metabolite of Lys, on proteolysis in C2C12 cells. When C2C12 myotubes were incubated in serum-free medium containing Sac, the rate of proteolysis, which was evaluated by 3-methylhistidine released from C2C12 myotubes, and autophagy activity, which was assessed by amount of light chain 3-II, were suppressed. Sac stimulated Akt and mammalian target of rapamycin signaling, which was evaluated from eIF4E-binding protein 1 phosphorylation. The suppressive effects of Sac on proteolysis and autophagy were completely abolished by an Akt inhibitor. Therefore, we concluded that Sac suppresses autophagic-proteolysis through Akt as with Lys.
- リンク情報
- ID情報
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- DOI : 10.1007/s11010-015-2541-9
- ISSN : 0300-8177
- eISSN : 1573-4919
- PubMed ID : 26307368
- Web of Science ID : WOS:000364018200009