First, SNI model mice were created, and it was confirmed by the von Frey test that the mechanical withdrawal threshold decreased from the 4th day to the 21st day after the operation.FAI measurements of neural activity in the primary somatosensory cortex (S1) and spinal dorsal horn of the same mouse model showed that S1 showed enhancement over time, whereas spinal cord showed no enhancement but rather a decreasing trend. Next, the effect of inhibition of microglial activity by administration of minocycline on neuropathic pain was examined. Administration of minocycline to the same model mice was able to prevent a decrease in the mechanical withdrawal threshold. On the other hand, the neural activity measurement by FAI did not show the recovery of spinal cord response of SNI model mice by administration of minocycline.