2011年5月
Identification of a high molecular weight kininogen fragment as a marker for early gastric cancer by serum proteome analysis
JOURNAL OF GASTROENTEROLOGY
- 巻
- 46
- 号
- 5
- 開始ページ
- 577
- 終了ページ
- 585
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00535-010-0369-3
- 出版者・発行元
- SPRINGER JAPAN KK
Serum biomarkers currently available for gastric cancers are not sufficiently sensitive and specific.We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) to generate comparative peptide profiles of serum samples obtained from gastric cancer patients (n = 81) and age- and sex-matched healthy controls (n = 66).Because of initial screening and further validation, we found that the intensities of a 2209 m/z MS peak were increased in the preoperative sera obtained from gastric cancer patients, and we identified this peak, a 2209 Da peptide, as a high molecular weight (HMW) kininogen fragment. Receiver operating characteristic analyses showed that the area under the curve (AUC) for the 2209 Da peptide (AUC = 0.715) was greater than those for conventional tumor markers (carcinoembryonic antigen AUC = 0.593, carbohydrate antigen 19-9 AUC = 0.527) used for the detection of stage I gastric cancers. Inverse correlations were observed between the levels of intact HMW kininogen and the 2209 Da peptide, suggesting that the upregulation of some protease activities is responsible for the overproduction of a kininogen fragment in gastric cancer patients.Serum levels of the 2209 Da peptide identified in this study have a greater diagnostic ability than those of conventional tumor markers used for the early detection of gastric cancer.
- リンク情報
- ID情報
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- DOI : 10.1007/s00535-010-0369-3
- ISSN : 0944-1174
- eISSN : 1435-5922
- PubMed ID : 21298293
- Web of Science ID : WOS:000290542300002