論文

本文へのリンクあり
2022年4月

The Critical Role of MMP13 in Regulating Tooth Development and Reactionary Dentinogenesis Repair Through the Wnt Signaling Pathway

Frontiers in Cell and Developmental Biology
  • Henry F. Duncan
  • ,
  • Yoshifumi Kobayashi
  • ,
  • Yukako Yamauchi
  • ,
  • Angela Quispe-Salcedo
  • ,
  • Zhi Chao Feng
  • ,
  • Jia Huang
  • ,
  • Nicola C. Partridge
  • ,
  • Teruyo Nakatani
  • ,
  • Jeanine D’Armiento
  • ,
  • Emi Shimizu

10
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.3389/fcell.2022.883266

Matrix-metalloproteinase-13 (MMP13) is important for bone formation and remodeling; however, its role in tooth development remains unknown. To investigate this, MMP13-knockout (Mmp13−/−) mice were used to analyze phenotypic changes in the dentin–pulp complex, mineralization-associated marker-expression, and mechanistic interactions. Immunohistochemistry demonstrated high MMP13-expression in pulp-tissue, ameloblasts, odontoblasts, and dentin in developing WT-molars, which reduced in adults, with human-DPC cultures demonstrating a >2000-fold increase in Mmp13-expression during mineralization. Morphologically, Mmp13−/− molars displayed critical alterations in the dentin-phenotype, affecting dentin-tubule regularity, the odontoblast-palisade and predentin-definition with significantly reduced dentin volume (∼30% incisor; 13% molar), and enamel and dentin mineral-density. Reactionary-tertiary-dentin in response to injury was reduced at Mmp13−/− molar cusp-tips but with significantly more dystrophic pulpal mineralization in MMP13-null samples. Odontoblast differentiation-markers, nestin and DSP, reduced in expression after MMP13-loss in vivo, with reduced calcium deposition in MMP13-null DPC cultures. RNA-sequencing analysis of WT and Mmp13−/− pulp highlighted 5,020 transcripts to have significantly >2.0-fold change, with pathway-analysis indicating downregulation of the Wnt-signaling pathway, supported by reduced in vivo expression of the Wnt-responsive gene Axin2. Mmp13 interaction with Axin2 could be partly responsible for the loss of odontoblastic activity and alteration to the tooth phenotype and volume which is evident in this study. Overall, our novel findings indicate MMP13 as critical for tooth development and mineralization processes, highlighting mechanistic interaction with the Wnt-signaling pathway.

リンク情報
DOI
https://doi.org/10.3389/fcell.2022.883266
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85129690173&origin=inward 本文へのリンクあり
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https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85129690173&origin=inward
ID情報
  • DOI : 10.3389/fcell.2022.883266
  • eISSN : 2296-634X
  • SCOPUS ID : 85129690173

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