2020年2月
Identification of descarbonsildenafil in an adulterated dietary supplement and evaluation of its inhibitory activity for phosphodiesterase type 5 (PDE5)
Journal of the Food Hygienic Society of Japan
- 巻
- 61
- 号
- 1
- 開始ページ
- 34
- 終了ページ
- 40
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3358/shokueishi.61.34
© 2020 Food Hygienic Society of Japan. All rights reserved. Some illegal dietary supplements contain phosphodiesterase type 5 (PDE5) inhibitors, such as sildenafil, for exertingtherapeutic effects in erectile dysfunction. This is apparently dangerous, and thus, should be appropriately regulated. Identification of descarbonsildenafil was first reported in Singapore in a coffee sample labeled to exert male sexual performance enhancement effects. However, it is unclear whether the compound possesses PDE5 inhibitory activity. We encountered during our survey of dietary supplements, a sexual enhancement product commercially available in Tokyo, in which a peak presumed to be of descarbonsildenafil was detected by LC-UV and electro-spray ionization-tandem MS (ESI-MS/MS). The compound was isolated and identified as descarbonsildenafil with liquid chromatography-quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS), NMR, and X-ray crystal structural analysis. In addition, descarbonsildenafil showed PDE5 inhibitory activity in PDE5 inhibition assay, and its IC50 value for PDE5A1 was found to be 30 nmol/L. The results of INADEQUATE NMR and X-ray crystal structural analysis in this study provide information for the identification of descarbonsildenafil. Since this study indicates that this compound is a PDE5 inhibitor having adequate activity, it is regulated as a drug component in Japan.
- リンク情報
- ID情報
-
- DOI : 10.3358/shokueishi.61.34
- ISSN : 0015-6426
- PubMed ID : 32336717
- SCOPUS ID : 85084107355