論文

査読有り 国際誌
2018年5月8日

Epigenetic Regulation of Aldosterone Synthase Gene by Sodium and Angiotensin II.

Journal of the American Heart Association
  • Yoshimichi Takeda
  • ,
  • Masashi Demura
  • ,
  • Fen Wang
  • ,
  • Shigehiro Karashima
  • ,
  • Takashi Yoneda
  • ,
  • Mitsuhiro Kometani
  • ,
  • Atsushi Hashimoto
  • ,
  • Daisuke Aono
  • ,
  • Shin-Ichi Horike
  • ,
  • Makiko Meguro-Horike
  • ,
  • Masakazu Yamagishi
  • ,
  • Yoshiyu Takeda

7
10
記述言語
英語
掲載種別
DOI
10.1161/JAHA.117.008281

BACKGROUND: DNA methylation is believed to be maintained in adult somatic cells. Recent findings, however, suggest that all methylation patterns are not stable. We demonstrate that stimulatory signals can change the DNA methylation status around transcription factor binding sites and a transcription start site and activate expression of the aldosterone synthase gene (CYP11B2). METHODS AND RESULTS: DNA methylation of CYP11B2 was analyzed in aldosterone-producing adenomas, nonfunctioning adrenal adenomas, and adrenal glands and compared with the gene expression levels. CpG dinucleotides in the CYP11B2 promoter were found to be hypormethylated in tissues with high expression, but not in those with low expression, of CYP11B2. Methylation of the CYP11B2 promoter fused to a reporter gene decreased transcriptional activity. Methylation of recognition sequences of transcription factors, including CREB1, NGFIB (NR4A1), and NURR1 (NR4A2) diminished their DNA-binding activity. A methylated-CpG-binding protein MECP2 interacted directly with the methylated CYP11B2 promoter. In rats, low salt intake led to upregulation of CYP11B2 expression and DNA hypomethylation in the adrenal gland. Treatment with angiotensin II type 1 receptor antagonist decreased CYP11B2 expression and led to DNA hypermethylation. CONCLUSIONS: DNA demethylation may switch the phenotype of CYP11B2 expression from an inactive to an active state and regulate aldosterone biosynthesis.

リンク情報
DOI
https://doi.org/10.1161/JAHA.117.008281
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29739797
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6015301