論文

査読有り
2020年3月1日

Sulfuracidifex tepidarius gen. nov., sp. nov. and transfer of Sulfolobus metallicus Huber and Stetter 1992 to the genus Sulfuracidifex as Sulfuracidifex metallicus comb. nov.

International Journal of Systematic and Evolutionary Microbiology
  • Takashi Itoh
  • ,
  • Tatsuki Miura
  • ,
  • Hiroyuki D. Sakai
  • ,
  • Shingo Kato
  • ,
  • Moriya Ohkuma
  • ,
  • Tomonori Takashina

70
3
開始ページ
1837
終了ページ
1842
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1099/ijsem.0.003981
出版者・発行元
Microbiology Society

Two novel, strictly aerobic, sulfur-dependent, thermoacidophilic strains, IC-006T and IC-007, were isolated from a solfataric field at Hakone Ohwaku-dani, Kanagawa, Japan. Cells of the two strains were irregular cocci with a diameter of 1.0–1.8 µm. They were strict aerobes and grew in a temperature range between 45 and 69 °C (optimally at 65 °C) and a pH range between 0.4 and 5.5 (optimally at pH 3.5). They required sulfur or a reduced sulfur compound, and sulfur was oxidized to sulfate. They grew autotrophically or mixotrophically utilizing several sugars and complex organic substances as carbon sources. The DNA G+C content was 42.4 mol%. A comparison of the 16S rRNA gene sequences among members of the order <italic>
<named-content content-type="order">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.64" xlink:type="simple">Sulfolobales</ext-link>
</named-content>
</italic> indicated that they were closely related to <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.70" xlink:type="simple">Sulfolobus metallicus</ext-link>
</named-content>
</italic>, forming an independent lineage within this order. The two isolates and <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.70" xlink:type="simple">Sulfolobus metallicus</ext-link>
</named-content>
</italic> were also diffentiated based on their phenotypic properties from the other members of the order <italic>
<named-content content-type="order">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.64" xlink:type="simple">Sulfolobales</ext-link>
</named-content>
</italic>. Detailed comparisons of the phenotypic properties and DNA–DNA hybridization study illustrated that the two isolates belong to a species different from <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.70" xlink:type="simple">Sulfolobus metallicus</ext-link>
</named-content>
</italic>. On the basis of the phylogenetic and phenotypic comparisons, we propose a new genus and species, <italic>Sulfuracidifex tepidarius</italic> gen. nov., sp. nov. to accommodate strains IC-006T and IC-007. The type strain of <italic>Sulfuracidifex tepidarius</italic> is IC-006T (=JCM 16833T=DSM 104736T). In addition, <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.70" xlink:type="simple">Sulfolobus metallicus</ext-link>
</named-content>
</italic> should be transferred to the new genus as <italic>Sulfuracidifex metallicus</italic> comb. nov.: the type strain is Kra23T (=DSM 6482T=JCM 9184T=NBRC 15436T).

リンク情報
DOI
https://doi.org/10.1099/ijsem.0.003981
URL
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003981?crawler=true

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