論文

査読有り
2016年10月

Conformational plasticity of JRAB/MICAL-L2 provides "law and order" in collective cell migration

MOLECULAR BIOLOGY OF THE CELL
  • Ayuko Sakane
  • ,
  • Shin Yoshizawa
  • ,
  • Masaomi Nishimura
  • ,
  • Yuko Tsuchiya
  • ,
  • Natsuki Matsushita
  • ,
  • Kazuhisa Miyake
  • ,
  • Kazuki Horikawa
  • ,
  • Issei Imoto
  • ,
  • Chiharu Mizuguchi
  • ,
  • Hiroyuki Saito
  • ,
  • Takato Ueno
  • ,
  • Sachi Matsushita
  • ,
  • Hisashi Haga
  • ,
  • Shinji Deguchi
  • ,
  • Kenji Mizuguchi
  • ,
  • Hideo Yokota
  • ,
  • Takuya Sasaki

27
20
開始ページ
3095
終了ページ
3108
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1091/mbc.E16-05-0332
出版者・発行元
AMER SOC CELL BIOLOGY

In fundamental biological processes, cells often move in groups, a process termed collective cell migration. Collectively migrating cells are much better organized than a random assemblage of individual cells. Many molecules have been identified as factors involved in collective cell migration, and no one molecule is adequate to explain the whole picture. Here we show that JRAB/MICAL-L2, an effector protein of Rab13 GTPase, provides the "law and order" allowing myriad cells to behave as a single unit just by changing its conformation. First, we generated a structural model of JRAB/MICAL-L2 by a combination of bioinformatic and biochemical analyses and showed how JRAB/MICAL-L2 interacts with Rab13 and how its conformational change occurs. We combined cell biology, live imaging, computational biology, and biomechanics to show that impairment of conformational plasticity in JRAB/MICAL-L2 causes excessive rigidity and loss of directionality, leading to imbalance in cell group behavior. This multidisciplinary approach supports the concept that the conformational plasticity of a single molecule provides "law and order" in collective cell migration.

Web of Science ® 被引用回数 : 9

リンク情報
DOI
https://doi.org/10.1091/mbc.E16-05-0332
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27582384
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000385185000009&DestApp=WOS_CPL

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