2011年
On-chip incubation system for long-term microfluidic cell culture
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
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- 開始ページ
- 8404
- 終了ページ
- 8407
- 記述言語
- 英語
- 掲載種別
- 研究論文(国際会議プロシーディングス)
- DOI
- 10.1109/IEMBS.2011.6092073
We demonstrate the use of a microfluidic cell culture chip with Braille pin-driven pumping, capable of on-chip CO2 incubation that does not require an external chamber or gas supply. The proposed chip consists of a poly(dimethylsiloxane)(PDMS)-made microfluidic chip, flip-mounted on a glass slide, that contains a nested pair of cell culture media reservoirs and water-jacket, insulated by a permeable PDMS wall. By using 0.8 M sodium bicarbonate with 65 mM sodium carbonate as the water-jacket and placing on a 37 °C surface, the chip maintained osmolality shift and the pCO2 in the media reservoir stabilized within <
3 mmol/kg and 5.0% ± 0.2% over at least 24 hours. The incubation capabilities were demonstrated through microfluidic culture of CV-1 epithelial cells under an inverted microscope for at least 12 days. © 2011 IEEE.
3 mmol/kg and 5.0% ± 0.2% over at least 24 hours. The incubation capabilities were demonstrated through microfluidic culture of CV-1 epithelial cells under an inverted microscope for at least 12 days. © 2011 IEEE.
- リンク情報
- ID情報
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- DOI : 10.1109/IEMBS.2011.6092073
- ISSN : 1557-170X
- PubMed ID : 22256297
- SCOPUS ID : 84055222424