Papers

Peer-reviewed
2018

Enhanced Optical Collection of Micro- and Nanovesicles in the Presence of Gold Nanoparticles

ACS Omega
  • Mai Kuboi
  • ,
  • Nobuyuki Takeyasu
  • ,
  • Takashi Kaneta

Volume
3
Number
3
First page
2527
Last page
2531
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1021/acsomega.8b00033
Publisher
American Chemical Society

We describe a process for collecting micro- and nanovesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on a glass substrate that sandwiched a solution containing vesicles prepared using a phospholipid. The optical pressure generated at the surface of the vesicles pulled them into the center of the beam where they formed an aggregate on the glass surface. The vesicles prepared with a buffer solution were successfully collected via adsorption onto the glass surface, whereas the vesicles prepared with pure water exhibited no such tendency. The time required to collect a certain amount of vesicles was inversely proportional to their concentration. To enhance the collection efficiency, we added gold nanoparticles to the vesicle solution. The addition of gold nanoparticles into the solution reduced the collection time to one-tenth of that without it, and this was attributed to thermal mixing promoted by the heat generated by the absorption from the gold nanoparticles in the solution, as well as to an enhancement of light scattering induced by the gold nanoparticles. The optical collection of vesicles coupled with gold nanoparticles shows a promise for the collection of trace amounts of extracellular vesicles in biological fluids.

Link information
DOI
https://doi.org/10.1021/acsomega.8b00033
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000427939400007&DestApp=WOS_CPL
URL
http://orcid.org/0000-0001-7221-1606
ID information
  • DOI : 10.1021/acsomega.8b00033
  • ISSN : 2470-1343
  • ORCID - Put Code : 42213715
  • SCOPUS ID : 85042874705
  • Web of Science ID : WOS:000427939400007

Export
BibTeX RIS