J-GLOBAL         Last updated: Oct 15, 2019 at 23:24
Tottori University
Faculty of Agriculture
Job title
Master of Agriculture, Doctor of Agriculture(Kyoto University)

Research Areas


Academic & Professional Experience

Kyoto University, Research Assistant


Graduate School, Division of Agriculture, Kyoto University
Faculty of Agriculture, Kyoto University

Awards & Honors

Mar 2004
Mechanism of Avenanthramide Induction, 日本農薬学会賞(奨励賞)
Japanease Society of Pesticide Science, Society Awards 2004 (on high prospectiveness)
Biochemical and molecular characterization of orange- and tangerine-colored rice calli. Plant Biotechnology, Vol. 32, No. 3, 193-203 (2015), Best Paper Award, Japanese Society for Plant Cell and Molecular Biology
Winner: Atsushi Ishihara, Kazuhiko Ohishi, Tetsuya Yamada, Mari Shibata-Hatta, Yuko Arai-Kichise, Satoru Watanabe, Hirofumi Yoshikawa, Kyo Wakasa*
Apr 2019
Induced phenylamide accumulation in response to pathogen infection and hormone treatment in rice (Oryza sativa).Bioscience, Biotechnology, and Biochemistry 82: 407-416 (2018), The Excellent Paper Award Published in Bioscience, Biotechnology, & Biochemistry 2018, Japan Society for Bioscience, Biotechnology, and Agrochemistry
Winner: Morimoto, Noriko; UENO, Kotomi; Teraishi, Masayoshi; Okumoto, Yutaka; Mori, Naoki; ISHIHARA*

Published Papers

Eltigani SA, Eltayeb MM, Ishihara A, Arima J
Journal of Food Biochemistry   in press    [Refereed]
Natural variation in the expression and catalytic activity of a naringenin O-methyltransferase influences antifungal defenses in diverse rice cultivars.
Murata K, Kitano T, Yoshimoto R, Takata R, Ube N, Ueno K, Ueno M, Yabuta Y, Teraishi M, Holland CK, Jander G, Okumoto Y, Mori N, Ishihara A*
The Plant Journal   In press.    [Refereed]
Ube N, Harada D, Katsuyama Y, Osaki-Oka K, Tonooka T, Ueno K, Taketa S, Ishihara A*
Phytochemistry   167 112095   Nov 2019   [Refereed]
1) Inducible metabolites were analyzed in pathogen-infected wheat.
2)Phenylamides composed of cinnamic acid and oxygenated tryptamines were identified.
3) These phenylamides serve as phytoalexins in wheat.
4) Phenylamides of agmatine and putrescin...
Ishihara A,* Sugai N, Bito T, Ube N, Ueno K, Okuda Y, Fukushima-Sakuno E
Bioscience, Biotechnology, and Biochemistry   83(10) 1800-1806   Oct 2019   [Refereed]
Kariya K, Murata K, Kokubo Y, Ube N, Ueno K, Yabuta Y, Teraishi M, Okumoto Y, Mori N, Ishihara A*
Phytochemistry   166 112057   Sep 2019   [Refereed]
1) Oryzalexin A accumulating and deficient chemotypes were present in rice.
2) In the oryzalexin A deficient cultivars, KSL10 was not upregulated by UV light.
3) Oryzalexin A was acquired by a common ancestor of Oryzae rufipogon.
4)Oryzalexin A wa...
Ishihara A, * Ando K, Yoshioka A, Murata K, Kokubo Y, Morimoto N, Ube N, Yabuta Y, Ueno M, Tebayashi S, Ueno K, Osaki-Oka K
Journal of Pesticide Sciences   44(2) 89-96   May 2019   [Refereed]
Ishihara A,* Ashida C, Ube N, Abe M, Hiyoshi H, Umezu K, Endo N, Sotome K, Maekawa N, Nakagiri A, Osaki-Oka K, Ichiyanagi T, Ueno K
44(1) 9-14   Jan 2019   [Refereed]
Yabuta Y., Mukoyama, H., Kaneda, Y., Kimura, N., Bito, T., Ichiyanagi, T., Ishihara, A., Watanabe, F.
Bioscience, Biotechnology, and Biochemistry   82(9) 1584-1590   Sep 2018
Streptococcus mutans is a bacterium found in human oral biofilms (dental plaques) that is associated with the development of dental caries. Glucosyltransferases (GTFs) are key enzymes involved in dental plaque formation, and compounds that inhibit...
Nishiguchi, S., Murata, K., Ube, N., Ueno, K., Tebayashi, S., Teraishi, M., Okumoto, Y., Mori, N., Ishihara, A.
Journal of Pesticide Science   43 191-197   2018
日本きのこ学会誌   26 28-31   2018
Ishihara, A., Goto, N., Kikkawa, M., Ueno, M., Ushijima, S., Ube, N., Ueno, K., Osaki-Oka, K.
Journal of Pesticide Sciences   43 108-113   2018
In view of the possibility that spent mushroom substrates (SMSs) may be used as agricultural materials to prevent crop diseases, we examined the effect of treatment with a hot water extract from the SMS of Lentinula edodes on plant resistance to p...
Cell proliferation and migration mechanism of caffeoylserotonin and serotonin via Serotonin 2B receptor in human keratinocyte HaCaT cells.
Kim, H.-E., Cho, H., Ishihara, A., Kim, B., Kim, O.
BMB Reports   51 188-193   2018
Okada, R., Ichinose, S., Takeshita, K., Urayama, S., Fukuhara, T., Komatsu, K., Arie, T., Ishihara, A., Egusa, M, Kodama, M., Moriyama, H.
Virology   518 23-32   2018
Morimoto, N., Ueno, K., Teraishi, M., Okumoto, Y., Mori, N., Ishihara, A.
Bioscience, Biotechnology, and Biochemistry   82 407-416   2018
Rice plants accumulate various specialized metabolites, including phenylamides, in response to pathogen attack. We prepared 25 phenylamides, and developed a method of analyzing them by multiple reaction monitoring with liquid chromatography couple...
Ishihara, A., Ide, Y., Bito, T., Ube, N., Endo, N., Sotome, K., Maekawa, N., Ueno, K., Nakagiri, A.
Bioscience, Biotechnology, and Biochemistry   82 22-30   2018
Tyrosinase is the key enzyme that controls melanin formation in the human skin. We performed a screening of 96 extracts of mushroom cultures and fruiting bodies for examining their inhibitory activity against mushroom tyrosinase. The ethyl acetate...
Tanaka, K., Fukuda, M., Amaki, Y., Sakaguchi, T., Inai, K., Ishihara, A., Nakajima, H.
Pest Management Science   73 2419-2428   2017
Cho, H., Kim, O., Lee. Y., Kang, L.-J., Nguyen, C. N., Ishihara, A., Kim, H.-E.
Biochemical and Biophysical Research Communications   491 973-979   2017
Ube, N., Nishizaka, M., Ichiyanagi, T., Ueno, K., Taketa, S., Ishihara, A.
Phytochemistry   141 1-11   2017
Plants have developed defensive specialized metabolites over the course of evolution. In the genus Hordeum, which includes the important cereal crop barley, specialized metabolites such as hordatines, benzoxazinones, and gramine have been identifi...
Ishihara, A., Kumeda, R., Hayashi, N., Yagi, Y., Sakaguchi, N., Kokubo, Y., Ube, N., Tebayashi, S., Ueno, K.
Bioscience, Biotechnology, and Biochemistry   81 1090-1098   2017
The inducible metabolites were analyzed in barley leaves inoculated with Bipolaris sorokiniana, the causal agent of spot blotch of barley. HPLC analysis revealed that B. sorokiniana-infected leaves accumulated 4 hydrophilic compounds. They were pu...
Production of host-selective SV-toxins by Stemphylium sp. causing brown spot of european pear in Japan.
Tanahashi, M., Okuda, S., Miyazaki, E., Parada, R. Y., Ishihara, A., Otani, H., Osaki-Oka, K.
Journal of Phytopathology   165 189-194   2017
Kokubo, Y., Nishizaka, M., Ube, N., Yabuta, Y., Tebayashi, S., Ueno, K., Taketa, S., Ishihara, A.
Bioscience, Biotechnology, and Biochemistry   81 431-440   2017
The Poaceae is a large taxonomic group consisting of approximately 12,000 species and is classified into 12 subfamilies. Gramine and benzoxazinones (Bxs), which are biosynthesized from the tryptophan pathway, are well-known defensive secondary met...
Asai, T., Matsukawa, T., Ishihara, A., Shin'ichiro Kajiyama
Journal of Bioscience and Bioengineering   122 208-212   2016
Ishihara, A., Fukami, A., Matsuda, Y., Nakajima, H., Miyagawa, H.
Journal of Phytopathology   164 433-570   2016
Li L., Zheng, M., Long, H., Deng, G., Ishihara, A., Liu, F., Liang, J., Pan, Z., Yu, M.
Plant Molecular Biology Reporter   34 273-282   2016
Oka,K., Ishihara, A., Sakaguchi, N., Nishino, S., Parada, R. Y., Nakagiri, A. Otani, H.
Journal of Phytopathology   163 987-996   2015
Ishihara, A., Ohishi, K., Yamada, T., Shibata-Hatta, M., Arai-Kichise, Y., Watanabe, S., Yoshikawa, H., Wakasa, K.
Plant Biotechnology   32 193-203   2015
Tanaka, K., Amaki, Y., Ishihara, A., and Nakajima, H.
Journal of Agricultural and Food Chemistry   63 5344-5353   2015
日本きのこ学会誌   22 147-152   2015
Ishihara, A., Kojima, K., Fujita, T., Yamamoto, Y., Nakajima, H.
Bioscience, Biotechnology, and Biochemistry   78 1975-1983   2014
Avenanthramides are characteristic constituents of oat seeds. We analyzed the methanol extract of oat seeds by HPLC and detected three compounds 1, 2, and 3 eluted at retention times similar to avenanthramides. The three compounds were purified by...
Williams, B. B., Van Benschoten, A. H., Cimermancic, P., Donia, M. S., Zimmermann, M., Taketani, M., Ishihara, A., Kashyap, P. C., Fraser, J. S., and Fischbach, M. A.
Cell Host & Microbe   16 495-503   2014
Insect-induced daidzein, formononetin and their conjugates in soybean leaves.
Murakami, S., Nakata, R., Aboshi, T., Yoshinaga, N., Teraishi, M., Okumoto, Y., Ishihara. A., Morisaka, H., Huffaker, A., Schmelz, E. A. and Mori, N.
Metabolites   4 532-546   2014
Takeda, S., Tanaka, H., Shimada, A., Morita, T., Ishihara, A., Adilbish, A., Delgermaa, B., and Gungaa, O.
Journal of Veterinary Medical Science   76 839-846   2014
Taguchi, T., Ishihara, A., and Nakajima, H.
Mycotoxins   64 1-14   2014
Tanaka, K., Ishihara, A., and Nakajima, H.
Journal of Agricultural and Food Chemistry   62 1469-1476   2014
Dubouzet, J. D, Matsuda, F., Ishihara, A., Miyagawa, H. and Wakasa, K.
Plant Biotechnology Journal   11 1103-1111   2013
Suzuki, M., Nishida, N., Ishihara, A. and Nakajima, H.
Bioscience, Biotechnology, and Biochemistry   77 271-275   2013
Taguchi, T., Kozutsumi, D., Nakamura, R., Sato, Y., Ishihara, A. and Nakajima, H.
Bioscience, Biotechnology, and Biochemistry   77 138-144   2013
Muroi, A., Matsui, K., Shimoda, T., Kihara, H., Ozawa, R., Ishihara, A., Nishihara, M. and Arimura, G.
Scientific Reports   2 Article number: 689   2012
Suzuki, M., Sakuno, E., Ishihara, A., Tamura, J. and Nakajima, H.
Phytochemistry   75 14-20   2012
Uchihashi, K., Nakayashiki, H., Okamura, K., Ishihara, A., Tosa, Y., Park, P. and Mayama, S.
Physiological and Molecular Plant Pathology   76 173-181   2011
Ishihara, A., Nakao, T., Mashimo, Y., Murai, M., Ichimaru, N., Tanaka, C., Nakajima, H., Wakasa, K. and Miyagawa, H.
Phytochemistry   72 7-13   2011
Tryptophan-derived secondary metabolites, including serotonin and its hydroxycinnamic acid amides, markedly accumulate in rice leaves in response to pathogen attack. These compounds have been implicated in the physical defense system against patho...
Choi, J., Kim, H., Choi, Y., Ishihara, A., Back, K. and Lee, S.
Fitoterapia   81 1134-1141   2010
Matsuda, F., Ishihara, A., Takanashi, K., Morino, K., Miyazawa, H., Wakasa, K. and Miyagawa, H.
Plant Biotechnology   27 17-27   2010
Nakajima, H., Ishihara, A., Sawa, Y. and Sakuno, E.
Journal of Agricultural and Food Chemistry   58 2882-2885   2010
Koshiba, T., Kobayashi, M., Ishihara, A. and Matoh, T.
Plant and Cell Physiology   51 323-327   2010
Kang, K., Lee, K., Ishihara, A., Park, S., Kim, Y. S. and Back, K.
Scientia Horticulture   124 290-293   2010
Muroi, A., Ishihara, A., Tanaka, C., Ishizuka, A., Takabayashi, J., Miyoshi, H. and Nishioka, T.
Planta   230 517-527   2009
Sasai, H., Ishida, M., Murakami, K., Tadokoro, N., Ishihara, A., Nishida, R. and Mori, N.
Bioscience, Biotechnology, Biochemistry   73 1333-1338   2009
Izumi, Y., Kajiyama, S., Nakamura, R., Ishihara, A., Okazawa, A., Fukusaki, E., Kanematsu, Y. and Kobayashi, A.
Planta   229 931-943   2009
Kyo Wakasa, Atsushi Ishihara
Plant Biotechnology   26(5) 523-533   2009   [Refereed]
Ichimaru, N., Murai, M., Kakutani, N., Kako, J., Ishihara, A., Nakagawa, Y., Nishioka, Y., Yagi, T. and Miyoshi, H.
Biochemistry   47 10816-10826   2008
Induction of serotonin accumulation by feeding of rice striped stem borer in rice leaves.
Ishihara, A., Hashimoto, Y., Miyagawa, H. and Wakasa, K.
Plant Signaling & Behavior   3 714-716   2008
Ly, D., Kang, K., Choi, J., Ishihara, A., Back, K. and Lee, S.
Journal of Medicinal Food   1 385-389   2008
Ishihara, A., Hashimoto, Y., Tanaka, C., Dubouzet, J. G., Nakao, T., Matsuda, F., Nishioka, T., Miyagawa, H. and Wakasa, K.
The Plant Journal   54 481-495   2008
The upregulation of the tryptophan (Trp) pathway in rice leaves infected by Bipolaris oryzae was indicated by: (i) enhanced enzyme activity of anthranilate synthase (AS), which regulates metabolic flux in the Trp pathway; (ii) elevated levels of t...
Nomura, T., Ishizuka, A., Kishida, K., Islam, A. K. M. R., Endo, T. R., Iwamura, H. and Ishihara, A.
Genes & Genetic Systems   82 455-464   2007
Dubouzet, J. G., Ishihara, A., Matsuda, F., Miyagawa, H., Iwata, H. and Wakasa, K.
Journal of Experimental Botany   58 3309-3321   2007
Tebayashi,S., Horibata, Y., Mikagi, E., Kashiwagi, T., Mekuria, D. B., Dekebo, A., Ishihara, A. and Kim, C.
Bioscience, Biotechnology, Biochemistry   71 1521-1526   2007
Murai, M., Ishihara, A., Nishioka, T., Yagi, T. and Miyoshi, H.
Biochemistry   46 6409-6416   2007
Okazaki, Y., Ishizuka, A., Ishihara, A., Nishioka, T. and Iwamura, H.
Journal of Organic Chemistry   72 3830-3839   2007
Nomura, T., Ishihara, A., Iwamura, H. and Endo, T. R.
Phytochemistry   68 1008-1016   2007
Atsushi Ishihara, Fumio Matsuda, Hisashi Miyagawa, Kyo Wakasa
Metabolomics   3(3) 319-334   2007   [Refereed]
Ishihara, A., Asada, Y., Takahashi, Y., Yabe, N., Komeda, Y., Nishioka, T., Miyagawa, H. and Wakasa, K.
Phytochemistry   67 2349-2362   2006
Kang, S., Kang, K., Chung, G. C., Choi, D., Ishihara A., Lee D. and Back, K.
Plant Physiology   140 704-715   2006


研究トピックス きのこから有用物質を探すーきのこ抽出物ライブラリー作成と有用生理活性物質の探索−
菌蕈   64(5) 3-8   May 2018
48 9-10   Feb 2016
Journal of Pesticide Science   29(3) 217-219   2004

Books etc

Chapter 20. Involvement of tryptophan-pathway-derived secondary metabolism in the defense responses of grasses."Amino Acids in Higher Plants"
Atsushi Ishihara, Tetsuya Matsukawa, Taiji Nomura, Masayuki Sue, Akira Oikawa, Yozo Okazaki, Shinichi Tebayashi
CABI   Apr 2015   ISBN:13: 978-1780642635

Conference Activities & Talks

植物化学調節学会第53回大会   4 Nov 2018   
植物化学調節学会第53回大会   4 Nov 2018   
植物微生物研究会第28回研究交流会   20 Sep 2018   
日本きのこ学会第22回大会   2018   
日本農薬学会第43回大会   2018   


Bioorganic chemistry of plant defense mechanisms
1996 - 2000

Research Grants & Projects

Exploration plant defense activator in spent mushroom substrates
Project Year: 2018 - 2020    Investigator(s): 石原 亨
Studies on saprophytic, parasitic and symbiotic strategies depending on secondary metabolite production in necrotrophic plant pathogens
Project Year: 2017 - 2019    Investigator(s): 児玉 基一朗
Physiological and ecological roles of alkaloids produced by endophyte of luguminous species
Project Year: 2012 - 2014    Investigator(s): 石原 亨
New inhibitor for the functional analysis of tryptophan metabolism in plants
Project Year: 2009 - 2011    Investigator(s): 石原 亨
Project Year: 2006 - 2007    Investigator(s): 西岡 孝明


A Gene involved in synthesis of IGs and a mutant plant accumulating IGs at a high level