論文

査読有り
2015年10月

Involvement of AMPK in regulating slow-twitch muscle atrophy during hindlimb unloading in mice

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
  • Tatsuro Egawa
  • ,
  • Ayumi Goto
  • ,
  • Yoshitaka Ohno
  • ,
  • Shingo Yokoyama
  • ,
  • Akihiro Ikuta
  • ,
  • Miho Suzuki
  • ,
  • Takao Sugiura
  • ,
  • Yoshinobu Ohira
  • ,
  • Toshitada Yoshioka
  • ,
  • Tatsuya Hayashi
  • ,
  • Katsumasa Goto

309
7
開始ページ
E651
終了ページ
E662
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1152/ajpendo.00165.2015
出版者・発行元
AMER PHYSIOLOGICAL SOC

AMPK is considered to have a role in regulating skeletal muscle mass. However, there are no studies investigating the function of AMPK in modulating skeletal muscle mass during atrophic conditions. In the present study, we investigated the difference in unloading-associated muscle atrophy and molecular functions in response to 2-wk hindlimb suspension between transgenic mice overexpressing the dominant-negative mutant of AMPK (AMPK-DN) and their wild-type (WT) littermates. Male WT (n = 24) and AMPK-DN (n = 24) mice were randomly divided into two groups: an untreated preexperimental control group (n = 12 in each group) and an unloading (n = 12 in each group) group. The relative soleus muscle weight and fiber cross-sectional area to body weight were decreased by similar to 30% in WT mice by hindlimb unloading and by similar to 20% in AMPK-DN mice. There were no changes in puromycin-labeled protein or Akt/70-kDa ribosomal S6 kinase signaling, the indicators of protein synthesis. The expressions of ubiquitinated proteins and muscle RING finger 1 mRNA and protein, markers of the ubiquitin-proteasome system, were increased by hindlimb unloading in WT mice but not in AMPK-DN mice. The expressions of molecules related to the protein degradation system, phosphorylated forkhead box class O3a, inhibitor of kappa B alpha, microRNA (miR)-1, and miR-23a, were decreased only in WT mice in response to hindlimb unloading, and 72-kDa heat shock protein expression was higher in AMPK-DN mice than in WT mice. These results imply that AMPK partially regulates unloading-induced atrophy of slow-twitch muscle possibly through modulation of the protein degradation system, especially the ubiquitin-proteasome system.

Web of Science ® 被引用回数 : 29

リンク情報
DOI
https://doi.org/10.1152/ajpendo.00165.2015
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26244519
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000362237800005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1152/ajpendo.00165.2015
  • ISSN : 0193-1849
  • eISSN : 1522-1555
  • PubMed ID : 26244519
  • Web of Science ID : WOS:000362237800005

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