Apr, 2019 - Mar, 2022
Study for a molecular machinery of the host recognition by Clostridioides difficile phage to develop the advanced therapy
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
- Grant number
- 19K07560
- Japan Grant Number (JGN)
- JP19K07560
- Grant amount
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- (Total)
- 4,290,000 Japanese Yen
- (Direct funding)
- 3,300,000 Japanese Yen
- (Indirect funding)
- 990,000 Japanese Yen
Clostridioides difficile infection (CDI), which is caused by excessive proliferation of C. difficile, is one of the antibacterial drug-related diarrhea and enteritis. Therefore, it is important to develop a new and specific treatment method for C. difficile disinfection. The current study was focused on bacteriophage (phage) as a potential treatment. Phage binds specifically to the host bacterium and generates a bactericidal activity. In this study, the recombinant phage proteins were expressed and purified and the bindings between these proteins and C. difficile were examined using enzyme-linked immunosorbent assay (ELISA) and western blot analysis. The results of the study showed a dose- and a time-dependent binding.
- Link information
- ID information
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- Grant number : 19K07560
- Japan Grant Number (JGN) : JP19K07560