論文

査読有り
2017年7月1日

Anti-oxidative Activity of Hydrolysate from Rice Bran Protein in HepG2 Cells.

Biological & pharmaceutical bulletin
  • Chie Moritani
  • ,
  • Kayoko Kawakami
  • ,
  • Akiko Fujita
  • ,
  • Koji Kawakami
  • ,
  • Tadashi Hatanaka
  • ,
  • Seiji Tsuboi

40
7
開始ページ
984
終了ページ
991
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1248/bpb.b16-00971
出版者・発行元
PHARMACEUTICAL SOC JAPAN

Glutathione (GSH) is an ubiquitous thiol-containing tripeptide, which plays important roles in cellular protection from oxidative stress. In our search for a dietary source that can increase GSH levels, we discovered that a 24 h treatment of HepG2 cells with rice bran protein hydrolysate (RBPH), prepared by Umamizyme G-catalyzed hydrolysis, increased the GSH content in a dose-dependent manner. RBPH elevated the expression levels of γ-glutamylcysteine synthetase (γ-GCS), which constitutes the rate-limiting enzyme of GSH synthesis, and of another two enzymes, hemeoxygenase-1 (HO-1) and reduced nicotinamide adenine dinucleotide (phosphate): quinone oxidoreductase 1 (NQO1). This induction was preceded by the accumulation of nuclear factor erythroid 2-related factor 2 (Nrf2) inside the nucleus, which is a key transcription factor for the expression of the γ-GCS, HO-1, and NQO1. Pre-treatment of cells with RBPH produced a significant protective effect against cytotoxicity caused by H2O2 or ethanol. These results indicate that RBPH exerts a protective effect against oxidative stress by modulating GSH levels and anti-oxidative enzyme expression via the Nrf2 pathway.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1248/bpb.b16-00971
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28381791
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000404670100006&DestApp=WOS_CPL

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